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FLA Sigma

Escherichia coli Amino-terminal FLAG® Expression Kit

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Properties

form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C

Description

Principle

The E. coli Amino-Terminal FLAG® Expression Kit provides the specialized FLAG components needed to perform expression of a recombinant N-terminal FLAG fusion in Escherichia coli. The pFLAG-ATS (E5769) and pFLAG-MAC (E5644) Expression Vectors provide a choice of secreted or cytoplasmic expression of N-terminal FLAG fusion proteins, respectively, under control of the tac promoter. Also provided in the kit are controls for expression, detection, affinity purification and enterokinase removal of N-terminal FLAG.

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

pFLAG-ATS is a trademark of Sigma-Aldrich Co. LLC

pFLAG-MAC is a trademark of Sigma-Aldrich Co. LLC

Price and Availability

Standard component

Description

Product #

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ANTI-FLAG M1 monoclonal antibody 200 μg SDS F3040
ANTI-FLAG M2 afinity gel 1 mL SDS    
ANTI-FLAG M2 monoclonal antibody 200 μg SDS F3165
Enterokinase 50 U SDS E5144
FLAG peptide 4 mg SDS F3290
C-24 sequencing primer 1 μg SDS P7957
N-26 sequencing primer 1 μg SDS P7832
N-terminal FLAG–BAP control protein 100 μg SDS P7582
Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles
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