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FLAA Sigma

Adenosine 5′-triphosphate (ATP) Bioluminescent Assay Kit

for ATP quantitation

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

ATP Bioluminescent Assay Kit can be used to determine the viability of the Pneumocystis carinii fungus isolated from infected rat lung tissues.

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Biomedical Applications

Standard component

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FL-AAB Dilution buffer 1 mL/vial SDS    
FL-AAM Assay mix 1 mL/vial SDS    
FL-AAS ATP standard 1 mL/vial SDS    
Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 3316 9
WGK Germany 
3

Frequently Asked Questions

How many reactions can one do with Product FLAA, ATP Bioluminescent Assay Kit (one vial of FLAAM)?
When one reconstitutes the vial to 5 ml, as directed, one can perform 50 assays using 100 ul per test. If one dilutes 1:5 one can get 250 tests per kit. To view this information on the technical bulletin please click on the following link: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Bulletin/flaabul.Par.0001.File.tmp/flaabul.pdf
What is the sensitivity of Product FLAA, ATP Bioluminescent Assay Kit the assay mix?
With FLAAM diluted 1:5, one can easily measure 1 pmol ATP in 100 µl (10 nM) with 50 µl of the reagent.
What  is the stability of the Product FLAA, ATP Bioluminescent Assay Kit ATP stock solution?
The ATP standard stock solution (FLAAS) is stable for at least 24 hours at 0-5°C, or for over two weeks if stored frozen at -20°C.
Can Product FLAA, ATP Bioluminescent Assay Kit assay mix be used on cells and tissues?
This reagent measures only soluble ATP. Cells must be washed and then lysed to obtain a soluble ATP extract. Tissues must be homogenized and clarified to provide an extract. The supernatant solution can then be used for assay.
How can one check for inhibitors in the samples when using Product FLAA, ATP Bioluminescent Assay Kit?
One must add an amount of ATP from a standard solution equivalent to the ATP already found in the sample. If the signal is doubled, the absence of an inhibitor is indicated.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Peer-Reviewed Papers
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