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FLAGIPT1 Sigma

FLAG® Immunoprecipitation Kit

Synonym: Anti-ddddk, Anti-dykddddk

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Description

Application

Browse additional application references in our FLAG® Literature portal.

Features and Benefits

• Utilizes highly specific ANTI-FLAG M2 affinity gel
• No preliminary steps or calibrations
• 3X FLAG peptide provides efficient and gentle elution through direct competition

Antibody Bioguarantee
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Other Notes

All prodedures to be performed at 2-8°C.

General description

Sigma’s FLAG Immunoprecipitation kit allows a rapid and efficient immunoprecipitation and elution of an active FLAG-tagged protein. The immunoprecipitation is performed with the ANTI-FLAG M2 affinity gel, which is a highly specific monoclonal antibody covalently attached to agarose resin. The use of affinity resin allows an efficient binding of FLAG-tagged proteins without the need for preliminary steps and calibrations. The immunoprecipitated FLAG-tagged proteins can be efficiently eluted from the resin with acidic conditions or by competition with FLAG peptide. The immunoprecipitated proteins can be detected for their size, post-translational modifications, and interactions on gel electrophoresis, and by activity assays.

Legal Information

3xFLAG is a trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability


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Amplified Detection

Standard component

Description

Product #

Add to Cart

ANTI-FLAG M2 affinity gel 1 mL SDS A2220
FLAG-BAP™ control protein 50 μg SDS P7582
3X FLAG peptide 1 mg SDS F4799
Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

Related Products

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Product #

Description

Add to Cart

C2978 CelLytic M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization.
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