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FLMA Sigma

Mammalian Amino-terminal FLAG® Transient Expression Kit

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Related Categories Cell Biology, Expression/Translation, General Stem Cell Biology, Stem Cell Biology, Stem Cell Characterization More...
shipped in   wet ice
storage temp.   −20°C

Description

Principle

The Mammalian Amino-Terminal FLAG® Transient Expression Kit provides all of the specialized FLAG components needed to perform expression of a recombinant N-terminal FLAG fusion in mammalian cells. The pFLAG-CMV-1 (E7273) and pFLAG-CMV-2 (E7398) Expression Vectors allow transient expression of secreted or cytoplasmically expressed fusion proteins. Also provided in the kit are controls for expression, detection, and affinity purification.

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

pFLAG-CMV1 is a trademark of Sigma-Aldrich Co. LLC

Price and Availability

Standard component

Description

Product #

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ANTI-FLAG M2 affinity gel 1 mL SDS    
ANTI-FLAG M2 monoclonal antibody 200 μg SDS F3165
ANTI-FLAG M5 monoclonal antibody 200 μg SDS F4042
C-CMV-24 sequencing primer 1 μg SDS P5475
FLAG peptide 4 mg SDS F3290
Met-FLAG-BAP control protein 100 μg SDS P5975
pFLAG-CMV-1 expression vector (preprotrypsin leader) 10 μg SDS    
N-CMV-30 sequencing primer 1 μg SDS P5350
Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles
Related Products

replaced by

Product #

Description

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E7033 pFLAG-CMV-2 Expression Vector, shuttle vector for intracellular transient expression of N-terminal Met-FLAG
P5475 C-CMV-24 Sequencing Primer
F3290 FLAG® Peptide, lyophilized powder
P5975 Amino-terminal Met-FLAG-BAP Fusion Protein
F4042 Monoclonal ANTI-FLAG® M5 antibody produced in mouse, clone M5, purified immunoglobulin, buffered aqueous solution

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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