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FLMAS Sigma

Mammalian Amino-terminal FLAG® Stable Expression Kit

Kit includes pFLAG-CMV-3 (E6783) and pFLAG-CMV-4 (E7158)

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Properties

grade   for molecular biology
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C

Description

Components

• pFLAG-CMV-3a Expression Vector (E8770) 20 μg
• pFLAG-CMV-4a Expression Vector (E1775) 20 μg
• pFLAG-CMV-3-BAPa Control Plasmid (C3972) 20 μg
• pFLAG-CMV-4-BAPa Control Plasmid (C4722) 20 μg
• C-CMV-24 Reverse Sequencing Primer (P5475) 1 μg
• N-CMV-30 Forward Sequencing Primer (P5350) 1 μg
• ANTI-FLAG M2 Monoclonal Antibody (F3165) 200 μg
• ANTI-FLAG M5 Monoclonal Antibody (F4042) 200 μg
• ANTI-FLAG M2 Affinity Gel - Freezer Safe (A2220) 1 ml
• Met-FLAG-BAP Control Protein (P5975) 100 ug
• FLAG Peptide (F3290) 4 mg

Application

The pFLAG-CMV-3 and pFLAG-CMV-4 Expression Vectors are suitable for transient or stable expression of secreted or cytoplasmic N-terminal FLAG® fusion proteins.

General description

The Mammalian Amino-Terminal FLAG Stable Expression Kit provides the specialized FLAG® components needed for expression of a recombinant N-terminal FLAG fusion in mammalian cells. The pFLAG-CMV-3 and pFLAG-CMV-4 expression vectors encode the aminoglycoside phosphotransferase II gene (Neo) which confers resistance to aminoglycosides such as G418, allowing for selection of stable transfectants.

Vector Maps and Sequences

Principle

The FLAG® system is based on the FLAG marker octapeptide (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys). The FLAG sequence is hydrophilic and the last 5 amino acids (Asp-Asp-Asp-Asp-Lys) represent the target sequence of the protease enterokinase. FLAG fusion proteins are created by cloning the coding sequence of a protein of interest adjacent to the FLAG coding sequence in a FLAG expression vector, followed by expression in the appropriate host cell type.

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Introduction to Blue-White Screening

Blue-white screening is a rapid and efficient technique for the identification of recombinant bacteria. It relies on the activity of β-galactosidase, an enzyme occurring in E. coli, which cleaves lac...
Keywords: Antibiotics, Cloning, Culture media, Gene expression, Immunocytochemistry, Peptide synthesis, Polymerase chain reaction, Recombination, Sterilizations, transformation

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