• USA Home
  • G0298 - Anti-GIT1 (C-terminal) antibody produced in rabbit

G0298 Sigma

Anti-GIT1 (C-terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-G protein-coupled receptor kinase-interacting protein 1, Anti-GRK-interacting protein 1, Anti-p95Cat, Anti-p95PKL



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to G Proteins and Cyclic Nucleotides, Antibodies to Rho and Related Proteins,
species reactivity   rat, mouse
application(s)   immunoprecipitation: 15-20 μg using rat brain extracts (S1 fraction)
  western blot: 1-2 μg/mL using rat brain extracts (S1 fraction) and mouse brain (S1 fraction) extracts
clone   polyclonal
concentration   ~1.5 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt ~95 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... GIT1(28964)
mouse ... Git1(216963)
rat ... Git1(83709)
biological source   rabbit
conjugate   unconjugated



synthetic peptide corresponding to amino acids 725-741 of human GIT1, conjugated to KLH. The corresponding sequence is identical in human GIT1 isoforms 1and 2, and in rat and mouse GIT1.

General description

GIT1 (G protein-coupled receptor kinase interactor 1) is an adaptor protein with GTPase-activating protein activity for the ADP-ribosylating factor. It is also named as Cat-1, p95-APP1 and p95PKL.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.


Anti-GIT1 (C-terminal) antibody is suitable for immunoprecipitation at a concentration of 15-20μg using rat brain extract (S1 fraction) and western blot at a concentration of 1-2μg/mL using rat brain extracts (S1 fraction) and mouse brain (S1 fraction) extracts.

Features and Benefits

Antibody Bioguarantee
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Biochem/physiol Actions

GIT1 (G protein-coupled receptor kinase interactor 1) is predominantly distributed and exists in its full length form. It helps to control cellular responses through the activation of heterotrimeric G proteins. GITs can interact with several proteins including GRKs, ARF1, Rac1, Cdc42, PAK, MEK1, PLCg1, FAK, paxillin and PAK-exchange-interacting factor (PIX). It is also involved in receptor internalization. It helps in regulating membrane trafficking between the plasma membrane and endosomes, targeting ARFs to the ARF-GAP activity of GITs. Also it has been found to play a pathogenic role in Huntington′s disease.

Price and Availability

Antibody Explorer

Explore with Confidence
Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
Protocols & Articles

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?