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G0376 Sigma

β-Glucuronidase from bovine liver

Type B-3, ≥2,000,000 units/g solid

Synonym: β-D-Glucuronide glucuronosohydrolase

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Description

Packaging

500000, 1000000, 5000000 units in poly bottle

Unit Definition

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).

Application

β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.

Biochem/physiol Actions

β-glucuronidase catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.

Price and Availability

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
LZ8990000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

UHPLC/MS of Drugs and Metabolites in Urine following Enzymatic Hydrolysis

β-D-Glucuronide glucuronosohydrolase enzymes, commonly shortened to β-glucuronidase, play an important role in the analysis of biological fluids for the presence of metabolites for drug screening and...
Craig Aurand, Supelco; Kristen Brown, Pennsylvania State University
Reporter US Volume 33.2
Keywords: Carbohydrate metabolism, Derivatizations, Digestions, Enzyme activity, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolism, Metabolites, Sample preparations, Separation, Solid phase extractions, Solvents

Protocols

Enzymatic Assay of ß-Glucuronidase (EC 3.2.1.31) from Helix Pomatia and Bovine Liver

To standardize a procedure for Enzymatic Assay of b-Glucuronidase1 at Sigma-Aldrich St. Louis.
Keywords: Enzymology

Related Content

Analytical Enzyme Beta Glucuronidase

Sigma® β-Glucuronidases are routinely used for the enzymatic hydrolysis of glucuronides from urine1,2, plasma3,4, and other fluids5 prior to analysis by enzyme immunoassay, mass spectrometry, gas chr...
Keywords: Bacterial conjugations, Cancer, Chromatography, Gas chromatography, High performance liquid chromatography, Immunoassay, Mass spectrometry, PAGE, Purification

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
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N7006 4-Nitrophenyl β-D-glucopyranoside, ≥98% (TLC)
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