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G0501 Sigma

β-Glucuronidase from bovine liver

Type B-10, ~10,000 units/mg solid

Synonym: β-D-Glucuronide glucuronosohydrolase

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Description

Unit Definition

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).

Application

β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.

Biochem/physiol Actions

β-glucuronidase catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.

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Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
LZ8990000
Protocols & Articles

Protocols

Enzymatic Assay of ß-Glucuronidase (EC 3.2.1.31) from Helix Pomatia and Bovine Liver

To standardize a procedure for Enzymatic Assay of b-Glucuronidase1 at Sigma-Aldrich St. Louis.
Keywords: Enzymology

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Sigma® β-Glucuronidases are routinely used for the enzymatic hydrolysis of glucuronides from urine1,2, plasma3,4, and other fluids5 prior to analysis by enzyme immunoassay, mass spectrometry, gas chr...
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Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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