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G0763 Sigma

Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle

ammonium sulfate suspension, 80-120 units/mg protein (biuret)

Synonym: D-Glyceraldehyde 3-phosphate:NAD+ oxidoreductase (phosphorylating), GAPDH

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Properties

Related Categories 1.2.x.x Acting on aldehydes or oxo groups, 1.x.x.x Oxidoreductases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
form   ammonium sulfate suspension
foreign activity   3-Phosphoglyceric phosphokinase <0.05%
  triosephosphate isomerase, lactic dehydrogenase, myokinase, and pyruvate kinase <0.01%
storage temp.   2-8°C

Description

Unit Definition

One unit will reduce 1.0 μmole of 3-phosphoglycerate to D-glyceraldehyde 3-phosphate per min in a coupled system with 3-phosphoglyceric phosphokinase at pH 7.6 at 25 °C.

Physical form

Crystalline suspension in 2.6 M (NH4)2SO4, pH 7.5, containing 1 mM EDTA and 0.02 M 2-mercaptoethanol

Biochem/physiol Actions

Glyceraldehyde-3-phosphate dehydrogenase catalyzes the conversion of glyceraldehyde-3-phosphate into D-glycerate-1,3-bisphosphate as part of the glycolysis pathway.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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