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G0907 Sigma

Anti-Granulocyte-Macrophage Colony Stimulating Factor antibody produced in goat

affinity isolated antibody, lyophilized powder

Synonym: Anti-GM-CSF

Description

Immunogen

Purified recombinant rat GM-CSF expressed in E. coli.

General description

Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) is 18-22kD acidic glycoproteins that produce in vitro granulocyte and macrophage colonies from bone marrow precursor cells. It facilitates in regulating granulocyte and macrophage lineage populations at every stage of maturation. It also plays an essential role in inflammation and autoimmunity. Anti-Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) antibody can be used for neutralizing the biological activity of recombinant rat GM-CSF. Goat anti-GM-CSF antibody reacts specifically with recombinant rat GM-CSF. The product has also shown 30% cross-reactivity for recombinant mouse GM-CSF and 10% cross-reactivity for recombinant human GM-CSF.

Physical form

Lyophilized from a 0.2 μm filtered solution of phosphate buffered saline

Specificity

By immunoblotting and ELISA, the antibody shows 30% cross-reactivity with recombinant mouse GM-CSF and 10% cross-reactivity with recombinant human GM-CSF.

Application

Anti-Granulocyte-Macrophage Colony Stimulating Factor antibody can be used in western blotting at a working antibody concentration of 0.1-0.2 μg/ml. It can also be used in indirect ELISA (working concentration:-0.5-1.0 μg/ml).

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