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G1041 Sigma

EZBlue Gel Staining Reagent

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Description

Features and Benefits

• Premixed solution eliminates the time and effort required to prepare the stain
• Increased sensitivity ensures that low abundance proteins can be detected (as little as 5 ng)
• Rapid reaction significantly reduces the amount of time required to stain and rinse
• No solvent waste so you save time and money by eliminating hazardous material disposal

General description

Convenient, sensitive, and safe, EZBlue Coomassie Brilliant Blue G-250 colloidal protein stain improves protein electrophoresis results while significantly reducing staining time. Conveniently packaged, EZBlue requires no messy weigh-ups or additions of methanol or acid. As a colloidal stain, it reacts only with proteins, not the gel itself. Background staining is reduced, so protein bands can be visualized almost immediately. No destaining step is required, although a water wash may intensify bands and clarify the background. Most impressively, EZBlue is extremely sensitive, detecting as little as 5 ng of protein.

Other Notes

A ready-to-use Brilliant Blue G-250 based protein stain for "one step" ultrasensitive detection on polyacrylamide gels and PVDF membranes.

Application

EZBlue® Gel Staining Reagent has been used as a staining reagent in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).

Legal Information

EZBlue is a trademark of Sigma-Aldrich Co. LLC

Sigma-Aldrich is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability


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Protocols & Articles

Articles

Electrophoresis - Protein Staining Reagents

To meet the great diversity of protein analysis needs, Sigma offers a wide selection of protein visualization (staining) reagents. EZBlue™ and ProteoSilver™, designed specifically for proteomics, als...
Keywords: PAGE, Proteomics

Fluorescent Detection on SDS-PAGE Gels

Identify glycoproteins with superior selectivity. Allows fluorescent detection of glycoproteins directly in PAGE gels without blotting or use of antibodies.
Glycobiology Analysis Manual, 2nd Edition
Keywords: Applications, PAGE

Peer-Reviewed Papers
15

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