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G1146 Sigma

L-Glutamine–Penicillin–Streptomycin solution

with 200 mM L-glutamine, 10,000 U penicillin and 10 mg steptomycin/mL in 0.9% NaCl, sterile-filtered, BioReagent, suitable for cell culture

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Properties

Related Categories A - K, Antibacterial, Antibiotic Solutions, Antibiotics, Antibiotics A to Z,
sterility   sterile-filtered
product line   BioReagent
form   liquid
impurities   endotoxin, tested
suitability   suitable for cell culture
Mode of action   cell wall synthesis | interferes
  protein synthesis | interferes
antibiotic activity spectrum   Gram-negative bacteria
  Gram-positive bacteria
shipped in   dry ice
storage temp.   −20°C

Description

Components

Formulated with 200 mM L-glutamine, 10,000 units/ml penicillin, and 10 mg/ml streptomycin in 0.9% sodium chloride.

Application

Used to reduce or eliminate bacterial contamination in cell, especially primary cell, cultures. Recommended for use in cell culture applications at 10 ml/L.

Biochem/physiol Actions

Mode of Action: Glutamine is essential as an energy source for most mammalian cells in culture because of its amino acid carbon skeleton. Penicillin acts by inhibiting bacterial cell wall synthesis. Streptomycin inhibits prokaryote protein synthesis by preventing the transition from initiation complex to chain-elongating ribosome and causes miscoding.
Antimicrobial spectrum: Gram-negative and Gram-positive bacteria.

Protocols & Applications

Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

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Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Related Content

Antibiotic Selection Guide

1Application Key: CC = Callus Culture; PR = plant regeneration; EC = cotyledon, hypocotyl, or leaf disc culture; TS = transformant selection; SE = somatic embryogenesis; SSC = stem selection culture;...
Keywords: Antibiotics, Cell culture

Peer-Reviewed Papers
15

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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