EMAIL THIS PAGE TO A FRIEND
G1279 Sigma

G 418 disulfate salt

BioReagent, plant cell culture tested

Synonym: Antibiotic G418

Purchase

Properties

Related Categories A - K, Aminoglycosides, Antibacterial, Antibiotics, Antibiotics A to Z,
InChI Key   UHEPSJJJMTWUCP-NKCAIAFTSA-N
product line   BioReagent
form   powder
potency   ≥450 μg per mg (Dried basis)
application(s)   cell culture | plant: suitable
solubility   H2O: soluble50 mg/mL, clear to very slightly hazy, colorless to faintly yellow
suitability   plant cell culture tested
Featured Industry   Agriculture
Mode of action   protein synthesis | interferes
storage temp.   2-8°C

Description

Application

G418 disulfate salt is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells transfected with a iNOS promoter construct and neomycin resistance gene†. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 μg/mL or less while animal cells may require 300-500 μg/mL.

Caution

The product is stable for three years when stored at 2-8°C.

Packaging

1, 5 g in glass bottle

250 mg in glass bottle

Preparation Note

G418 powder is soluble in water at 50 mg/mL.

Protocols & Applications

Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

Price and Availability

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Protocols

Antibiotics - Plant Tissue Culture Protocol

In general, antibiotics require storage in a refrigerator or freezer. Aminoglycosides (e.g. kanamycin) are hygroscopic and should be stored in a desiccator. Storage of many powdered antibiotics at -2...
Keywords: Adsorption, Antibiotics, Antifungals, Condensations, Molecular biology

Related Content

Antibiotic Selection Guide

1Application Key: CC = Callus Culture; PR = plant regeneration; EC = cotyledon, hypocotyl, or leaf disc culture; TS = transformant selection; SE = somatic embryogenesis; SSC = stem selection culture;...
Keywords: Antibiotics, Cell culture

Plant Tissue Culture Products | Agriculture

Our combination of quality products, services and global business capabilities help you to maintain reagent consistency while ensuring suitability. Sigma-Aldrich allows you to standardize reagents ac...
Keywords: Antibiotics, Cell culture, Culture media, Gas chromatography, High performance liquid chromatography, Mass spectrometry, Vitamins, transformation

Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

related product

Product #

Description

Add to Cart

08168 Timestrip Plus 8 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?