G 418 disulfate salt plant cell culture tested, BioReagent

Properties

Related Categories	A - K, Aminoglycosides, Antibacterial, Antibiotics, Antibiotics A to Z, Antibiotics G-M, Antibiotics and Antimycotics, Antibiotics by Application, Antineoplastic and Immunosuppressive Antibiotics, Chemical Structure Class, Genetic Marker Selection, Interferes with Protein Synthesis, Mechanism of Action, Plant Biotechnology, Plant Tissue Culture, Spectrum of Activity More...
product line	BioReagent
suitability	plant cell culture tested
storage temp.	2-8°C

Description

Biochem/physiol Actions

G 418 blocks polypeptide synthesis by interfering with ribosomal function and inhibiting protein elongation.

Mode of Action: Blocks polypeptide synthesis by inhibiting protein elongation. For use in the selection and maintenance of eukaryotic cells stably transfected with neomycin resistance genes.

Other Notes

Aminoglycoside antibiotic similar in structure to gentamicin, neomycin and kanamycin.

Application

G 418 is a Micromonospora-produced aminoglycoside antibiotic with activity against protozoa and helminths¹. G 418 disulfate salt is used for selection and maintenance of prokaryotic and eukaryotic cells transfected with a iNOS promoter construct and neomycin resistance gene².

Protocols & Applications

Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

Price and Availability

Related Papers

1. Antibiotic G-418, a New Micromonospora-Produced Aminoglycoside with Activity Against Protozoa and Helminths: Fermentation, Isolation, and Preliminary Characterization G. H. Wagman, R. T. Testa, et al. Antimicrob. Agents Chemother. 6, 144-149, (1974)

Documents

Certificate of Analysis

Certificate of Origin

G1279 - Product Information Sheet (24 KB)

Plant Biotech Guide (179 KB )

Safety Information

Symbol	GHS08
Signal word	Danger
Hazard statements	H317-H334
Precautionary statements	P261-P280-P342 + P311
Personal Protective Equipment	Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Hazard Codes (Europe)	Xn
Risk Statements (Europe)	42/43
Safety Statements (Europe)	22-36/37-45
WGK Germany	3

Technical information & documentation associated with this product is available in the Safety & Documentation tab.

Papers

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1. Antibiotic G-418, a New Micromonospora-Produced Aminoglycoside with Activity Against Protozoa and Helminths: Fermentation, Isolation, and Preliminary Characterization G. H. Wagman, R. T. Testa, et al. Antimicrob. Agents Chemother. 6, 144-149, (1974)

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2. Orazipone Inhibits Activation of Inflammatory Transcription Factors Nuclear Factor-B and Signal Transducer and Activator of Transcription 1 and Decreases Inducible Nitric-Oxide Synthase Expression and Nitric Oxide Production in Response to Inflammatory Stimuli Outi Sareila, Mari Hämäläinen, et al. J. Pharmacol. Exp. Ther. 324, 858-866, (2008)

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Regulated expression of human interferon β1 gene after transduction in to cultured mouse and rabbit cells. Canaani, D., and Berg, P. Proc. Natl. Acad. Sci. U. S. A. 79, 5166-5170, (1982)

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Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. Southern, P., and Berg, P. J. Mol. Biol. 1, 327-341, (1982)

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Selective elimination of fibroblasts from cultures of normal human melanocytes. Halaban, R., and Alfano, F. In Vitro Cell. Dev. Biol. 20, 447-450, (1984)

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A new antibiotic with known resistance factors, G418, inhibits plant cells. Ursic, D., et al. Biochem. Biophys. Res. Commun. 101, 1031-1037, (1981)

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G-418, an elongation inhibitor of 80 S ribosomes. Bar-Nun, S., et al. Biochim. Biophys. Acta 741, 123-127, (1983)

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Stable transfer of genes into mammalian cells. Kingston, R, Ausubel, F. et al. Short Protocols in Molecular Biology 2nd ed., New York , (1992), 9.14