|Related Categories||Attachment Factors, Attachments Factors, Cell Culture, Core Bioreagents, Life Science Reagents for Cell Culture,|
|concentration||2% in H2O, tissue culture grade|
|surface coverage||100‑200 μg/cm2|
|suitability||suitable for cell culture|
Derived from lime-cured tissue bovine skin
Applications include using gelatin coating cell culture plates to improve cell attachment for a variety of cell types, addition to PCR to help stabilize Taq DNA polymerase, and use as a blocking reagent in Western blotting, ELISA, and immunohistochemistry.
Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular matrices, present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue and Type B gelatin is derived from lime-cured tissue.
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gel strength ~225 g Bloom, Type B
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From our library of Articles, Sigma-Aldrich presents Gelatin Cell Attachment Protocol
BioFiles 2008, 3.8, 10.
Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular weights, present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, bones, etc. in wat...
BioFiles 2008, 3.8, 10.
Keywords: Biomaterials, Cell attachment, Cell culture, Culture media, Enzyme-linked immunosorbent assay, Hemostatics, Immunohistochemistry, Pharmaceutical, Polymerase chain reaction, Western blot
Traditionally, cancer has been viewed as a disease in which environmental or endogenous events induce mutations to critical oncogenes and tumor suppressor genes within a normal cell. The clinical man...
BioFiles 2008, 3.5, 4.
Keywords: Angiogenesis, Apoptosis, Asymmetric synthesis, Cancer, Catalysis, Cell culture, Cell division, Cell proliferation, Clinical, DNA replication, Degradations, Eliminations, Environmental, Gastrointestinal, Gene expression, Growth factors, Ligands, Metabolic Pathways, Phosphorylations, Poisons, Transcription, Transfection, transformation
Synthesis and characterization of fluorescence resonance energy transfer-based nanoprobes by coating CdTe QDs with rhodamine B in gelatin nanoparticles. Li C, Wang X, Ye C, et al. J. Nanosci. Nanotechnol. 13(6), 4330-3, (2013)
Sub-micronic capsules based on gelatin and poly(maleic anhydride-alt-vinyl acetate) obtained by interfacial condensation with potential biomedical applications. Iurea DM, Peptu CA, Chailan JF, et al. J. Nanosci. Nanotechnol. 13(6), 3841-50, (2013)
Treatment with basic fibroblast growth factor-incorporated gelatin hydrogel does not exacerbate mechanical allodynia after spinal cord contusion injury in rats. Furuya T, Hashimoto M, Koda M, et al. J. Spinal Cord Med. 36(2), 134-9, (2013)
Generation of priming mesenchymal stem cells with enhanced potential to differentiate into specific cell lineages using extracellular matrix proteins. Han NR, Yun JI, Park YH, et al. Biochem. Biophys. Res. Commun. 436(3), 413-7, (2013)
Fluorometric immunocapture assay for the specific measurement of matrix metalloproteinase-9 activity in biological samples: application to brain and plasma from rats with ischemic stroke. Hawkins KE, DeMars KM, Yang C, et al. Mol. Brain 6, 14, (2013)
Enzyme-responsive copper sulphide nanoparticles for combined photoacoustic imaging, tumor-selective chemotherapy and photothermal therapy. Zha Z, Zhang S, Deng Z, et al. Chem. Commun. 49(33), 3455-7, (2013)
Solution growth of spherulitic rod and platelet calcium phosphate assemblies through polymer-assisted mesoscopic transformations. Kosma VA and Beltsios KG Mater. Sci. Eng. C. Mater. Biol. Appl. 33(4), 2175-91, (2013)
Polyethylene glycol-modified gelatin/polylactic acid nanoparticles for enhanced photodynamic efficacy of a hypocrellin derivative in vitro. Babu A, Periasamy J, Gunasekaran A, et al. J. Biomed. Nanotechnol. 9(2), 177-92, (2013)
Treatment of neural anosmia by topical application of basic fibroblast growth factor-gelatin hydrogel in the nasal cavity: an experimental study in mice. Nota J, Takahashi H, Hakuba N, et al. JAMA Otolaryngol. Head Neck Surg. 139(4), 396-400, (2013)
Enhanced mechanical strength and biocompatibility of electrospun polycaprolactone-gelatin scaffold with surface deposited nano-hydroxyapatite. Jaiswal AK, Chhabra H, Soni VP, et al. Mater. Sci. Eng. C. Mater. Biol. Appl. 33(4), 2376-85, (2013)
PCL and PCL-gelatin nanofibers as esophageal tissue scaffolds: optimization, characterization and cell-matrix interactions. Kuppan P, Sethuraman S, and Krishnan UM J. Biomed. Nanotechnol. 9(9), 1540-55, (2013)
Bi-layer composite dressing of gelatin nanofibrous mat and poly vinyl alcohol hydrogel for drug delivery and wound healing application: in-vitro and in-vivo studies. Jaiswal M, Gupta A, Agrawal AK, et al. J. Biomed. Nanotechnol. 9(9), 1495-508, (2013)
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