G1393 Sigma

Gelatin solution

Type B, 2% in H2O, tissue culture grade, sterile, BioReagent, suitable for cell culture

Properties

Related Categories Attachment Factors, Attachments Factors, Cell Culture, Core Bioreagents, Life Science Reagents for Cell Culture,
sterility   sterile
type   Type B
product line   BioReagent
concentration   2% in H2O, tissue culture grade
surface coverage   100‑200 μg/cm2
impurities   endotoxin, tested
suitability   suitable for cell culture
storage temp.   2-8°C

Description

Preparation Note

Derived from lime-cured tissue bovine skin

Application

Applications include using gelatin coating cell culture plates to improve cell attachment for a variety of cell types, addition to PCR to help stabilize Taq DNA polymerase, and use as a blocking reagent in Western blotting, ELISA, and immunohistochemistry.

General description

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular matrices, present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue and Type B gelatin is derived from lime-cured tissue.

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