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G1393 Sigma

Gelatin solution

Type B, 2% in H2O, tissue culture grade, sterile, BioReagent, suitable for cell culture

Properties

Related Categories Attachment Factors, Attachments Factors, Cell Culture, Core Bioreagents, Life Science Reagents for Cell Culture,
sterility   sterile
type   Type B
product line   BioReagent
concentration   2% in H2O, tissue culture grade
surface coverage   100‑200 μg/cm2
impurities   endotoxin, tested
suitability   suitable for cell culture
storage temp.   2-8°C

Description

Components

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue. Type B is derived from lime-cured tissue.

Application

This product is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.

Gelatin has been used in many applications. It has use in coating cell culture to improve attachment of cells, being added to PCR to stabilize Taq DNA, as a blocking reagent in Western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology. As a biocompatible polymer, it has used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications. It has also been used to study long-chain fatty acid-induced changes in gene expression in neonatal cardiac monocytes and to study to test mobilization of capillary endothelium in vitro induced by effectors of angiogenesis in vivo.

Caution

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Preparation Note

This product is derived lime-cured tissue bovine skin, and is a 2% solution in water.

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