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G1663 Sigma

Glycogen Synthase Kinase 3β, Histidine-tagged from rabbit

≥90% (SDS-PAGE), recombinant, expressed in E. coli

Synonym: GSK-3β, Kinase FA, Tau protein kinase I

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Description

Reconstitution

Reconstitution to 100 μl with deionized water produces a solution in 20 mM Tris, pH 7.5, 2 mM EDTA, 2 mM EGTA, 1 mM DTT, 5% sucrose, 0.1% Brij® L23, 1 μg/ml leupeptin, and 0.1 mM AEBSF.

Unit Definition

One unit will transfer one pmole of phosphate from ATP to phosphatase inhibitor 2 per min at pH 7.5 at 30 °C.

Application

Glycogen Synthase Kinase 3β (GSK3β) has been used
• to study GSK3β cleavage by calpain
• to perform motility assay on squid axoplasms
• for the pre-incubation of progesterone receptor A during in vitro ubiquitination assay

Biochem/physiol Actions

Glycogen Synthase Kinase 3β (GSK3β) is responsible for rapid cyclin D1 turnover by phosphorylating it on Thr-286. It functions as a negative regulator of Wnt signaling during embryonic development cell proliferation in adult tissues. This protein phosphorylates and regulates multiple proteins, such as glycogen synthase, insulin receptor substrate-1, axin and APC (adenomatous polyposis coli). This protein is also shown to interact with multiple proteins, which are linked with Alzheimer’s disease (AD).

General description

Glycogen Synthase Kinase 3β (GSK3β) is one of the two isoforms of GSK3 enzyme, both of which are encoded by different genes. This protein is predominantly cytoplasmic, but is also found to be localized to nucleus and mitochondria. It is a protein-serine kinase. This protein contains a protein kinase domain in its core, and is composed of 482 amino acids with a molecular weight of 46,712Da. It shows a wide range of tissue expression with the highest expression in brain.

Legal Information

Brij is a registered trademark of Croda International PLC

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Insulin Signaling and Energy Homeostasis

Glucose metabolism is regulated by the opposing actions of insulin and glucagon. Insulin is released from pancreatic ß cells in response to high blood glucose levels and regulates glucose metabolism ...
Linda Stephenson, Ph.D.
Biofiles v6 n4, 2011
Keywords: Apoptosis, Biological processes, Carboxylations, Catalog, Diabetes, Gene expression, Gluconeogenesis, Glycolysis, Hormones, Metabolism, Metabolites, Transcription, Transduction, Type

Peer-Reviewed Papers
15

References

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