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G1767 Sigma

Glycogen from Mytilus edulis (Blue mussel)

for DNA precipitations

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Properties

Related Categories Carrier Molecules, DNA & RNA Purification, Molecular Biology, Plant Biotechnology, Plant Molecular Biology,
grade   for molecular biology
form   liquid
packaging   vial of 1 mL
concentration   19-22 mg/mL
storage temp.   −20°C

Description

Principle

Glycogen is an inert carrier that traps nucleic acids. It forms a precipitate with DNA/RNA while being insoluble in ethanol solution. Due to the manufacturing process, there is low risk of contaminating nucleic acids in the glycogen. This product is tested for DNase, RNase, and NICKase, but not nucleic acids.

Application

Suitable for
• precipitation of DNA in the presence of ammonium acetate and ethanol or isopropanol
• recovery of tRNA, yeast RNA, sonicated DNA and oligonucleotides as short as 8 base pairs

Features and Benefits

• product provided is 1 mL total volume
• precipitates even small amounts of DNA and RNA present in dilute solutions
• does not interfere with OD 260/280 readings

General description

Glycogen is a branched polymer of glucose synthesized by animal cells for energy storage and release. It is constructed of predominantly α1→4 glycosidic bonds with branches created through α1→6 glycosidic bonds.

Glycogen is a branched polymer of glucose that coprecipitates nucleic acids during isolation and purification.

Price and Availability


Microcon Centrifugal Filters

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Glycogen from Mytilus edulis (Blue mussel)

Sigma’s Molecular Biology Grade Glycogen is a prepared solution of glycogen from mussel in sterile redistilled water at a concentration of approx. 20 mg per ml. This preparation is purified to remove...
Keywords: DNA purification, Molecular biology, Precipitation, Purification

Peer-Reviewed Papers
15

References

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