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G1890 Sigma

Gelatin from porcine skin

powder, gel strength ~300 g Bloom, Type A, BioReagent, for electrophoresis, suitable for cell culture

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Properties

Related Categories 3D Printing Materials for Research and Development, Attachment Factors, Attachments Factors, Cell Culture, Core Bioreagents,
grade   for electrophoresis
type   Type A
product line   BioReagent
form   powder
surface coverage   100‑200 μg/cm2
suitability   suitable for cell culture
storage temp.   room temp

Description

Components

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue. Type B is derived from lime-cured tissue.

Application

This product is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives. Gelatin has been used in many applications. It has use in coating cell culture to improve attachment of cells, being added to PCR to stabilize Taq DNA, as a blocking reagent in Western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology. As a biocompatible polymer, it has used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications. In the pharmaceutical industry, geltan can be used as a suspending and encapsulating agent, among other applications.

Caution

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Packaging

1 kg in poly bottle

100, 500 g in poly bottle

Preparation Note

This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. This product has a gel strength of approximately 300.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
RTECS 
LX8580000
Protocols & Articles

Articles

3D Printing of Carbon Fiber-Reinforced Composites

3D printing is a type of additive manufacturing that can be used to rapidly fabricate components with highly customizable geometries, most typically using a layer-by-layer fabrication process. 3D pri...
Zhenyu Bo* (Ph.D Candidate at Northwestern University) and Jia Choi*, PhD, Product Manager


*Materials Science Product Management Team, MilliporeSigma, Milwaukee, WI.
Keywords: Deposition, Nanomaterials, Nanotubes

3D-Cell culture: An overview of advanced tools and techniques

Cell culture techniques are ubiquitous in areas of developmental biology, drug discovery, regenerative medicine and protein production. Since the introduction of cell culture techniques, cells have b...
Keywords: Alkylations, Apoptosis, Biomaterials, Cell culture, Cell proliferation, Clinical, Cytotoxins, Drug discovery, Gene expression, Metabolism, Pharmaceutical

Cancer Stem Cells: New Targets for Cancer Therapy

Interested in receiving more content like this? Join over 8,000 of your peers who have already signed up for the Cell Informer newsletter.
Keywords: Cancer, Cell attachment, Cell culture, Cell proliferation, Gastrointestinal, Gene expression, Transcription, transformation

Gelatin Cell Attachment Protocol

From our library of Articles, Sigma-Aldrich presents Gelatin Cell Attachment Protocol
George Sitterley
BioFiles 2008, 3.8, 10.
Keywords: Sterilizations

Gelatin

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular weights, present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, bones, etc. in wat...
George Sitterley
BioFiles 2008, 3.8, 10.
Keywords: Biomaterials, Cell attachment, Cell culture, Culture media, Enzyme-linked immunosorbent assay, Hemostatics, Immunohistochemistry, Pharmaceutical, Polymerase chain reaction, Veterinary, Western blot

Three-Dimensional Printing of Tissue Engineering Scaffolds

Helena N. Chia, Ph.D.,1* Benjamin M. Wu, D.D.S, Ph.D.1,2* 1Department of Bioengineering, Henry Samueli School of Engineering University of California, Los Angeles, CA 90095 USA 2Division of Advanced ...
Keywords: Biomaterials, Ceramics, Chromatin immunoprecipitation, Deposition, Diagnostic Imaging, Diffusion, Growth factors, Materials Science, Melting, Polymerization reactions, Solvents

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Peer-Reviewed Papers
15

References

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