• USA Home
  • G1N10 - GenElute Mammalian Genomic DNA Miniprep Kits

EMAIL THIS PAGE TO A FRIEND
G1N10 Sigma

GenElute Mammalian Genomic DNA Miniprep Kits

sufficient for 10 purifications

Synonym: Gen Elute, Mammalian Genomic DNA Miniprep

Purchase

Description

Features and Benefits

• Expected yield: 25 μg from 2 x 106 cultured cells; 30 μg from 25 mg of tissue
• Elution volume: 200 - 400 μl
• Time required: 20 min after lysis
• A260/A280 ratio: 1.6 - 1.9
• Mechanical homogenization required: No

Other Notes

For additional information, please see www.sigma-aldrich.com/genomicdna.

Application

The purified genomic DNA is ready for immediate use in downstream applications such as:
• restriction endonuclease digestions
• PCR
• Southern blots
• sequencing reactions
• cloning
• ligation

Principle

The starting materials are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. Addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.

General description

The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient way to isolate pure, high molecular weight DNA from a variety of mammalian sources. These kits use a silica-based membrane, specially selected for genomic DNA purification, in a convenient spin column format.

Isolate pure genomic DNA from cultured cells, tissues (including rodent tails), and fresh whole blood or white blood cells. The kit combines the advantages of silica binding with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Price and Availability


Microcon Centrifugal Filters

Discover the Milli-Q® IQ 7000 ultrapure water system

Kit component also available separately

Description

Product #

Add to Cart

Column Preparation Solution SDS C2112
Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology SDS P2308
RNase A solution SDS R6148

Kit component only

Description

Product #

Add to Cart

Resuspension Solution SDS    
Lysis Solution T SDS    
Lysis Solution C SDS    
Wash Solution Concentrate SDS    
Elution Solution (10 mM Tris-HCl, 0.5 mM EDTA, pH 9.0) SDS    
Nucleic Acid Binding Column SDS    
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
Flash Point(F) 
483.8 °F
Flash Point(C) 
251 °C
Protocols & Articles

Articles

GenomePlex® Whole Genome Amplification Kit: A High-Throughput Approach for Rapidly Amplifying Genomic DNA from a Variety of Biological Materials

Genomic characterization has become the starting point for understanding biological systems. In many instances, this process is hampered by insufficient quantities of genomic DNA, especially for rare...
Keywords: Amplification, Genomics, Polymerase chain reaction, Sequencing, Whole genome amplification

Introduction to Nucleic Acid Electrophoresis

Introduction Agarose gel electrophoresis for DNA Agarose gel electrophoresis for RNA Polyacrylamide gel electrophoresis for DNA Reference
Keywords: AGE, Buffers, Degradations, Electrophoresis, Environmental, Gel electrophoresis, PAGE, Purification, Separation, Size-exclusion chromatography

Introduction to Southern and Northern Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is known as blotting. Fragments of DNA and RNA molecules separated by gel electrophoresis are trans...
Keywords: AGE, Buffers, Digestions, Electrophoresis, Filtration, Gel electrophoresis, Nucleic acid hybridization, Precipitation, Purification, Scintillation, Separation, Sterilizations

Sample Purification & Quality Assessment - PCR Technologies Guide

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RN...
Keywords: AGE, Absorption, Amplification, Bio-Analytical, Buffers, Capillary electrophoresis, Cell disruption, Centrifugation, Chromatin immunoprecipitation, DNA purification, Degradations, Detection methods, Detergents, Digestions, Electrophoresis, Extinction coefficient, Filtration, Gel electrophoresis, Gene expression, Indicators, Lab-on-a-chip, Molecular biology, Nucleic acid hybridization, Nucleic acid purification, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Purification, RNA purification, Separation, Spectroscopy, Transcription, Ultraviolet-Visible spectroscopy

Protocols

Animal Tissue DNA-Extraction & WGA Amplification Protocol

Animal tissue is a common source of material when performing genetic analysis. The protocol below is a simple method of extracting DNA from the animal sample. Once the DNA has been isolated, it can t...

Buccal DNA Extraction & WGA Amplification Protocol

This protocol provides a simple and convenient method to isolate, amplify, and purify genomic DNA from buccal swabs. Buccal swabs are a convenient method of acquiring a DNA sample. Once the DNA is is...

Experienced User Protocol | GenElut Mammalian Genomic DNA Miniprep Kit

For experienced users, an abbreviated protocol is listed below. For data, images, troubleshooting and other information, please see the full length protocol page.
Keywords: PAGE

FFPE Tissue

Archived Formalin-fixed, Paraffin-embedded (FFPE) tissue samples are invaluable resources for profiling gene expression and studying a variety of diseases. Since the archived DNA is usually available...
Keywords: Amplification, Cell disruption, DNA purification, Diseases, Evaporation, Gene expression, Molecular biology, Purification, Whole genome amplification

GenElute Mammalian Genomic DNA Miniprep Kit Protocol

Sigma’s GenElute Mammalian Genomic DNA Miniprep Kit provides a simple and convenient way to isolate pure genomic DNA from a variety of cultured cells, tissues (including rodent tails), and fresh whol...
Keywords: AGE, Amplification, Anticoagulants, Cell disruption, Centrifugation, DNA purification, Degradations, Digestions, Electrophoresis, Evaporation, Gel electrophoresis, Molecular biology, Nucleic acid denaturation, Phase transitions, Polymerase chain reaction, Precipitation, Purification, Sample preparations, Sequencing

Related Content

Genome-wide Screen for miRNA Targets Using the MISSION® Target ID Library [VIDEO]

The MISSION Target ID Library is designed to assist in discovery and identification of microRNA (miRNA) targets. The MISSION Target ID Library is a plasmid-based, genome-wide cDNA library cloned into...
Keywords: PAGE, Sequencing

Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

recommended

Product #

Description

Add to Cart

459836 Ethyl alcohol, Pure, 200 proof, anhydrous, ≥99.5%
W4502 Water, Molecular Biology Reagent

related product

Product #

Description

Add to Cart

G1N70 GenElute Mammalian Genomic DNA Miniprep Kits, sufficient for 70 purifications
D9281 Lambda DNA EcoR I Hind III Digest, for DNA electrophoresis
L3287 Escort IV Transfection Reagent, Lipid reagent for transfecting mammalian and insect cells
T6400 Tris-Borate-EDTA buffer, 5× Concentrate
G1N350 GenElute Mammalian Genomic DNA Miniprep Kits, sufficient for 350 purifications
R6148 RNase A solution
C2112 Column Preparation Solution
93021-001 DNAstable®, Tube kit, for room temp preservation of DNA
93221-001 RNAstable®, Tube Kit, for room temp preservation of RNA
T0956 Sigma Universal Transfection Reagent, Polymer reagent for transfecting common mammalian cell lines
A9434 Ammonium chloride, for molecular biology, suitable for cell culture, ≥99.5%
SRE0005 Proteinase K from Tritirachium album, Reagents designed and manufactured under cGMP controls suitable for use in an IVD application.
P2308 Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology
E1510 Ethidium bromide solution, BioReagent, for molecular biology, 10 mg/mL in H2O
A9539 Agarose, BioReagent, for molecular biology, low EEO

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?