GenElute^™ Mammalian Genomic DNA Miniprep Kits

Features and Benefits

• Typical DNA yields of 25 μg
• Preparation time is only 20 minutes after lysis
• Purified genomic DNA has A₂₆₀/A₂₈₀ ratios between 1.6 and 1.9
• No need for mechanical homogenization

Other Notes

The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient way to isolate pure, high molecular weight DNA from a variety of mammalian sources. These kits use a silica-based membrane, specially selected for genomic DNA purification, in a convenient spin column format.

For additional information, please see www.sigma-aldrich.com/genomicdna.

Application

The purified genomic DNA is ready for immediate use in downstream applications such as restriction endonuclease digestions, PCR, southern blots, and sequencing reactions.

General description

The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient way to isolate pure, high molecular weight DNA from a variety of mammalian sources. These kits use a silica-based membrane, specially selected for genomic DNA purification, in a convenient spin column format.

Isolate pure genomic DNA from a variety of cultured cells, tissues (including rodent tails), and fresh whole blood or white blood cells. The kit combines the advantages of silica binding with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.

The starting materials are lysed in a chaotropic salt containing solution to insure the thorough denaturation of macromolecules. Addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A₂₆₀/A₂₈₀ ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC