|Related Categories||Amplification, Cell Biology, Core Bioreagents, DNA & RNA Purification, Functional Profiling of Stem Cells,|
|usage||sufficient for 350 purifications|
|storage temp.||room temp|
• Expected yield: 25 μg from 2 x 106 cultured cells; 30 μg from 25 mg of tissue
• Elution volume: 200 - 400 μl
• Time required: 20 min after lysis
• A260/A280 ratio: 1.6 - 1.9
• Mechanical homogenization required: No
For additional information, please see www.sigma-aldrich.com/gen
The purified genomic DNA is ready for immediate use in downstream applications such as:
• restriction endonuclease digestions
• Southern blots
• sequencing reactions
The starting materials are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. Addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.
The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient way to isolate pure, high molecular weight DNA from a variety of mammalian sources. These kits use a silica-based membrane, specially selected for genomic DNA purification, in a convenient spin column format.
Isolate pure genomic DNA from cultured cells, tissues (including rodent tails), and fresh whole blood or white blood cells. The kit combines the advantages of silica binding with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.
GenElute is a trademark of Sigma-Aldrich Co. LLC
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|Lysis Solution T|
|Lysis Solution C|
|Wash Solution Concentrate|
|Elution Solution (10 mM Tris-HCl, 0.5 mM EDTA, pH 9.0)|
|Nucleic Acid Binding Column|
|Collection Tubes, 2.0 mL capacity|
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Certificate of Analysis
Certificate of Origin
For experienced users, an abbreviated protocol is listed below. For data, images, troubleshooting and other information, please see the full length protocol page.
Sigma’s GenElute Mammalian Genomic DNA Miniprep Kit provides a simple and convenient way to isolate pure genomic DNA from a variety of cultured cells, tissues (including rodent tails), and fresh whol...
Sigma’s GenElute™ Blood Genomic DNA Kit provides a simple and convenient way to isolate pure genomic DNA from fresh or aged (older than 24 hours) whole blood. The kit combines the advantages of silic...
Keywords: AGE, Addition reactions, Amplification, Anticoagulants, Catalog, Cell disruption, Centrifugation, Digestions, Electrophoresis, Evaporation, Gel electrophoresis, Help, Instructions, Molecular biology, Nucleic acid denaturation, Phase transitions, Polymerase chain reaction, Precipitation, Purification, Sequencing
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|G1N70||GenElute™ Mammalian Genomic DNA Miniprep Kits, sufficient for 70 purifications|
|D9281||Lambda DNA EcoR I Hind III Digest, for DNA electrophoresis|
|T6400||Tris-Borate-EDTA buffer, 5× Concentrate|
|G1N10||GenElute™ Mammalian Genomic DNA Miniprep Kits, sufficient for 10 purifications|
|R6148||RNase A solution|
|C2112||Column Preparation Solution|
|A9434||Ammonium chloride, for molecular biology, suitable for cell culture, ≥99.5%|
|SRE0005||Proteinase K from Tritirachium album, Suitable for manufacturing of diagnostic kits and reagents|
|P2308||Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology|
|E1510||Ethidium bromide solution, BioReagent, for molecular biology, 10 mg/mL in H2O|
|A9539||Agarose, For routine use|
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