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G1N350 Sigma

GenElute Mammalian Genomic DNA Miniprep Kits

sufficient for 350 purifications

Synonym: Gen Elute, Mammalian Genomic DNA Miniprep

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Description

Features and Benefits

• Expected yield: 25 μg from 2 x 106 cultured cells; 30 μg from 25 mg of tissue
• Elution volume: 200 - 400 μl
• Time required: 20 min after lysis
• A260/A280 ratio: 1.6 - 1.9
• Mechanical homogenization required: No

Other Notes

For additional information, please see www.sigma-aldrich.com/genomicdna.

Application

The purified genomic DNA is ready for immediate use in downstream applications such as:
• restriction endonuclease digestions
• PCR
• Southern blots
• sequencing reactions
• cloning
• ligation

Principle

The starting materials are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. Addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.

General description

The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient way to isolate pure, high molecular weight DNA from a variety of mammalian sources. These kits use a silica-based membrane, specially selected for genomic DNA purification, in a convenient spin column format.

Isolate pure genomic DNA from cultured cells, tissues (including rodent tails), and fresh whole blood or white blood cells. The kit combines the advantages of silica binding with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Price and Availability


D-Tube Dialyzers

Microcon Centrifugal Filters

All labs need water

Kit component also available separately

Description

Product #

Add to Cart

Column Preparation Solution SDS C2112
Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology SDS P2308
RNase A solution SDS R6148

Kit component only

Description

Product #

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Resuspension Solution SDS    
Lysis Solution T SDS    
Lysis Solution C SDS    
Wash Solution Concentrate SDS    
Elution Solution (10 mM Tris-HCl, 0.5 mM EDTA, pH 9.0) SDS    
Nucleic Acid Binding Column SDS    
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 3316 9
Protocols & Articles

Articles

Introduction to Nucleic Acid Electrophoresis

Introduction Agarose gel electrophoresis for DNA Agarose gel electrophoresis for RNA Polyacrylamide gel electrophoresis for DNA Reference
Keywords: AGE, Buffers, Degradations, Electrophoresis, Environmental, Gel electrophoresis, PAGE, Purification, Separation, Size-exclusion chromatography

Introduction to Southern and Northern Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is known as blotting. Fragments of DNA and RNA molecules separated by gel electrophoresis are trans...
Keywords: AGE, Buffers, Digestions, Electrophoresis, Filtration, Gel electrophoresis, Nucleic acid hybridization, Precipitation, Purification, Scintillation, Separation, Sterilizations

Protocols

Experienced User Protocol | GenElut Mammalian Genomic DNA Miniprep Kit

For experienced users, an abbreviated protocol is listed below. For data, images, troubleshooting and other information, please see the full length protocol page.
Keywords: PAGE

GenElute Mammalian Genomic DNA Miniprep Kit Protocol

Sigma’s GenElute Mammalian Genomic DNA Miniprep Kit provides a simple and convenient way to isolate pure genomic DNA from a variety of cultured cells, tissues (including rodent tails), and fresh whol...
Keywords: AGE, Amplification, Anticoagulants, Cell disruption, Centrifugation, DNA purification, Degradations, Digestions, Electrophoresis, Evaporation, Gel electrophoresis, Molecular biology, Nucleic acid denaturation, Phase transitions, Polymerase chain reaction, Precipitation, Purification, Sample preparations, Sequencing

GenElute™ Blood Genomic DNA Kit Protocol (NA2010, NA2020)

Sigma’s GenElute™ Blood Genomic DNA Kit provides a simple and convenient way to isolate pure genomic DNA from fresh or aged (older than 24 hours) whole blood. The kit combines the advantages of silic...
Keywords: AGE, Addition reactions, Amplification, Anticoagulants, Cell disruption, Centrifugation, Digestions, Electrophoresis, Evaporation, Gel electrophoresis, Molecular biology, Nucleic acid denaturation, Phase transitions, Polymerase chain reaction, Precipitation, Purification, Sequencing

Peer-Reviewed Papers
15

References

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Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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