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G2279 Sigma

Monoclonal Anti-β-COP antibody produced in mouse

clone M3A5, ascites fluid

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to COP Vesicles, Antibodies to Cell and Organelle Proteins,
species reactivity   human, rabbit, hamster, canine, monkey, bovine, rat, kangaroo rat, chicken, goose
application(s)   immunocytochemistry: suitable
  immunoprecipitation: suitable
  indirect immunofluorescence: 1:20 using cultured Chinese hamster ovary (CHO) cells
clone   M3A5, monoclonal
antibody form   ascites fluid
isotype   IgG1
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... COPB1(1315)
rat ... Copb1(114023)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

microtubule-associated protein from goose brain.

General description

The coatomer (approx. 550kDa) consists of proteins designated α-, β-, γ-, and δ-COP, together with substoichiometric amounts of several other proteins.

Specificity

The antibody recognizes an epitope shared by β-COP (110 kDa) found in most tissue culture lines, and by a doublet of brain microtubule-associated protein (MAP2, 270-300 kDa). The antibody stains a reticular structure in the perinuclear area of non-neuronal cells (the periphery of Golgi complex and a population of coatomers scattered throughout the cytoplasm) in tissues from different species and cell processes, as well as cell bodies in chicken brain neuronal cells. It has been used for studies on the effects of various agents that influence energy status, disrupt the Golgi complex, or alter the activity of G-proteins or small GTP-binding proteins on the cellular localization of β-COP. The antibody may be used for the immunoaffinity purification of the protein.

Application

Monoclonal Anti-β-COP antibody produced in mouse may be used for the localization of β-COP using immunoprecipitation, immunocytochemistry, immunoblotting. This antibody may be used along with other antibodies to Golgi proteins to study the role and relationships of this protein in the cell.

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Biochem/physiol Actions

COPs (coatomer proteins) contain adaptin-like, complex and are transiently attached to the vesicles involved in transport within the Golgi complex and possibly between the rough ER and Golgi complex. β-COP has a molar mass of 110kDa and its primary structure is homologous to the β-adaptin component of clathrin-coated vesicles.

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