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  • G2404 - Monoclonal Anti-Golgi 58K Protein/Formiminotransferase Cyclodeaminase (FTCD) antibody produced in mouse

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G2404 Sigma

Monoclonal Anti-Golgi 58K Protein/Formiminotransferase Cyclodeaminase (FTCD) antibody produced in mouse

clone 58K-9, ascites fluid

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cytoplasm,
species reactivity   monkey, hamster, bovine, mouse, human, pig, canine, rat, kangaroo rat
application(s)   electron microscopy: suitable
  indirect immunofluorescence: 1:50 using cultured CHO cells
  western blot: 1:5,000 using whole rat liver extract
clone   58K-9, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 58 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... FTCD(10841)
mouse ... Ftcd(14317)
rat ... Ftcd(89833)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

Golgi 58K protein from rat liver.

General description

FTCD is a homooctamer with its subunits arranged in a planar ring. It is predominantly expressed in the liver. Apart from being associated with the Golgi apparatus, it is detectable in the supernatant cytosolic fraction, and may also be localized to cytoplasmic vesicles.

Specificity

The antibody recognizes an epitope located on the microtubule-binding peripheral Golgi membrane 58 kDa protein. It is also useful for studies on the effect of microtubule-perturbing agents on the Golgi apparatus.

Application

Monoclonal Anti-Golgi 58K Protein/Formiminotransferase Cyclodeaminase (FTCD) antibody produced in mouse is suitable for electron microscopy, indirect immunofluorescence at a working dilution of 1:50 using cultured CHO cells and indirect immunoblotting at 1:5000 working dilution using whole rat liver extract. It is also useful for the localization of Golgi 58K protein using immunoblotting, dot blot, electron microscopy, and immunocytochemistry.
The antibody was used:
• for the analysis of distribution of the 58K9 protein exclusively localized in the Golgi
• as a primary antibody in the immunofluorescence analysis in studies related to functioning of nuclear envelope protein TMEM209 in lung carcinoma cells, tracking TG2 (Transglutaminase Type 2) transport in renal tubular epithelial cells, binding of TRADD (TNFR-associated death domain protein) to TNF-R1 at the plasma membrane and localization of Wilson disease protein in the Golgi apparatus
• in immunoprecipitation studies

Features and Benefits

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Biochem/physiol Actions

Golgi 58K protein is associated with the Golgi apparatus peripherally and has been identified as a version of FTCD (Formiminotransferase Cyclodeaminase). It is a bifunctional metabolic enzyme involved in conversion of histidine to glutamic acid. It acts as a channel for the transport of one-carbon units from formiminoglutamate to the folate pool. It also interacts with vimentin subunits and with polymerized vimentin filaments. Autosomal recessive disorder glutamate formiminotransferase deficiency is caused by defects in FTCD.

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