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G2500 Sigma

Gelatin from porcine skin

gel strength 300, Type A

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Properties

Related Categories 3D Printing Materials for Research and Development, Adhesives, Cell Biology, Core Bioreagents, Gelatin,
Quality Level   PREMIUM
type   Type A
gel strength   ~300 g Bloom
solubility   H2O: soluble50 mg/mL
suitability   suitable for cell culture

Description

Components

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue. Type B is derived from lime-cured tissue.

Caution

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Packaging

1 kg in poly bottle

100, 500 g in poly bottle

Preparation Note

This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. This product has a gell strength of 300 and is soluble in water at 50 mg/mL.

Application

This product is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.

Gelatin was used for coating cell culture to improve attachment of cells and was added to PCR to help stabilize Taq DNA. It was used as a blocking reagent in Western blotting, ELISA, and immunohistochemistry. Gelatin can also be used as a component of media for species differentiation in bacteriology. Gelatin is a biocompatible polymer and has been used as delivery vehicle for the release of bioactive molecules and in the generation of scaffolds for engineering applications. Gelatin was used in the murine lethality assay, which is used to examine the ability of SMAbs to neutralize the effects of BoNT/A1 in vivo.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
RTECS 
LX8580000
Protocols & Articles

Articles

3D Printing of Carbon Fiber-Reinforced Composites

3D printing is a type of additive manufacturing that can be used to rapidly fabricate components with highly customizable geometries, most typically using a layer-by-layer fabrication process. 3D pri...
Zhenyu Bo* (Ph.D Candidate at Northwestern University) and Jia Choi*, PhD, Product Manager


*Materials Science Product Management Team, MilliporeSigma, Milwaukee, WI.
Keywords: Deposition, Nanomaterials, Nanotubes

Peer-Reviewed Papers
15

References

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