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G25150 Sigma

Sephadex® G-25

Medium

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Description

Application

Sephadex® G-25 is a gel filtration medium used in affinity chromatography, protein chromatography and gel filtration chromatography. Sephadex® G-25 has been used to study the acceleration of diabetic wound healing and the treatment of epithelial damage.

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Sephadex is a registered trademark of GE Healthcare

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Coarse

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Safety Information

WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Peer-Reviewed Papers

References

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A Comprehensive Study of Genic Variation in Natural Populations of Drosophila melanogaster. IV. Mitochondrial DNA Variation and the Role of History ZIS. Selection in the Genetic Structure of Geographic Populations. Hale, L.R., and Singh, R.S. Genetics 129, 103-117, (1991)

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A Caenorhabditis elegans dauer-inducing pheromone and an antagonistic component of the food supply. Golden, J.W., and Riddle, D.L. J. Chem. Ecol. 10(8), 1265-1280, (1984)

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Rate constants for intramolecular electron-transfer reactions of ruthenium-modified histidine mutants of cytochrome b2. Lloyd, E., et al. Dalton Trans., 675-681, (1994)

Electrochemiluminescence Enzyme Immunoassays for TNT and Pentaerythritol Tetranitrate. Wilson, R., et al. Anal. Chem. 75, 4244-4249, (2003)

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Preparation and characterization of a pentaammineruthenium(III) derivative of horse heart ferricytochrome c. Yocom, K.M., et al. Proc. Natl. Acad. Sci. U. S. A. 79, 7052-7055, (1982)

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DNA Display I. Sequence-Encoded Routing of DNA Populations. Halpin, D.R., and Harbury, P.B. PLoS Biol. 2(7), e173, (2004)

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A Monoclonal-Antibody-Based Radioimmunoassay for Measurement of Protein C in Plasma. Howard, P.R., et al. Clin. Chem. 34(2), 324-330, (1988)

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Characterization of Endometrial Receptors for Ovine Trophoblast Protein-1 during the Estrous Cycle and Early Pregnancy in Sheep. Knickerbocker, J.J., and Niswender, G.D. Biol. Reprod. 40, 361-369, (1989)

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Isolation of a trypsin inhibitor from Echinodorus paniculatus seeds by affinity chromatography on immobilized Cratylia mollis isolectins. Paiva, P.M., et al. Bioresour. Technol. 88, 75-79, (2003)

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Separation of Motile Populations of Spermatozoa Prior to Freezing Is Beneficial for Subsequent Fertilization In Vitro: A Study with Various Mouse Strains. Szczygiel, M.A., et al. Biol. Reprod. 67, 287-292, (2002)

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Identification of major common extracellular proteins secreted by Aeromonas salmonicida strains isolated from diseased fish Fyfe, L., et al. Appl. Environ. Microbiol. 53(4), 722-726, (1987)

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Factor Xa Interacts With Two Sites on Monocytes With Different Functional Activities. Worfolk, L.A., et al. Blood 80(8), 1989-1997, (1992)

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Heterogeneity of apolipoprotein E epitope expression on human lipoproteins: importance for apolipoprotein E function. Krul, E.S., et al. J. Lipid Res. 29, 1309-1325, (1988)

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Acceleration of diabetic-wound healing with PEGylated rhaFGF in healing-impaired streptozocin diabetic rats. Huang, Z., et al. Wound Repair Regen. 19, 633-644, (2011)

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Chemical modification of recombinant human keratinocyte growth factor 2 with polyethylene glycol improves biostability and reduces animal immunogenicity. Huang, Z., et al. J. Biotechnol. 142, 242-249, (2009)

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A method for specific chemical modification of gamma-carboxyglutamic acid residues in proteins. Wright, S.F., et al. Anal. Biochem. 139(1), 82-90, (1984)

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Use of dual wavelength spectrophotometry and continuous enzymatic depletion of oxygen for determination of the oxygen binding constants of hemoglobin. Larsen, T.M., et al. Anal. Biochem. 197(1), 231-246, (1991)

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Characterization of the Role of Lysine 110 of NADH-Cytochrome b5 Reductase in the Binding and Oxidation of NADH by Site-directed Mutagenesis. Strittmatter, P., et al. J. Biol. Chem. 267(28), 20164-20167, (1992)

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Isolation of queuine from bovine amniotic fluid. Reyniers, J.P., and Farkas, W.R. Anal. Biochem. 130(2), 427-430, (1983)

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Membrane permeability of fructose-1,6-diphosphate in lipid vesicles and endothelial cells. Ehringer, W.D., et al. Mol. Cell Biochem. 210(1-2), 35-45, (2000)

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Stimulation of vitamin K-dependent carboxylation by pyridoxal-5'-phosphate. Dubin, A., et al. Biochem. Biophys. Res. Commun. 88(3), 1024-1029, (1979)

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[Chemical constituents of Salvia chinensis]. Gao JF, Ding L, Zhang P, et al. Zhongguo Zhong Yao Za Zhi 38(10), 1556-9, (2013)

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[Chemical constituents of Conioselinum vaginatum]. Li XB, Qi YD, Liu HT, et al. Zhongguo Zhong Yao Za Zhi 38(10), 1543-7, (2013)

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[Flavonoids from Humulus lupulus]. Zhang WK, Wang SB, Fu CY, et al. Zhongguo Zhong Yao Za Zhi 38(10), 1539-42, (2013)

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[Chemical constituents of Rhododendron seniavinii]. Wang QQ, Zhang Y, Ye WC, et al. Zhongguo Zhong Yao Za Zhi 38(3), 366-70, (2013)

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[Chemical constituents of Gentiana rhodantha]. Chen Y, Wang GK, Wu C, et al. Zhongguo Zhong Yao Za Zhi 38(3), 362-5, (2013)

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[Chemical constituents from cell cultures of Morus alba]. Tao XY, Zhang DW, Chen RD, et al. Zhongguo Zhong Yao Za Zhi 37(24), 3738-42, (2012)

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[Chemical constituents of Pilea cavaleriei subsp. cavaleriei]. Ren HC, Qin RD, Zhang QY, et al. Zhongguo Zhong Yao Za Zhi 37(17), 2581-4, (2012)

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Dispersive mixing and intraparticle partitioning of protein in size-exclusion chromatographic refolding Ding, Y., et al. J. Chromatogr. A. 1218, 8503-8510, (2011)

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Synergistic enhancement of cancer therapy using a combination of docetaxel and photothermal ablation induced by single-walled carbon nanotubes. Wang L Int. J. Nanomedicine 6, 2641-52, (2011)

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The chicken type III GnRH receptor homologue is predominantly expressed in the pituitary, and exhibits similar ligand specificity to the type I receptor. Joseph, N.T., et al. J. Endocrinol. 202, 179, (2009)

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Comparative study on preparative methods of DC-Chol/DOPE liposomes and formulation optimization by determining encapsulation efficiency. Yang S, Chen J, Zhao D, et al. Int. J. Pharm. 434(1-2), 155-60, (2012)

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Comparative study of chromatographic medium-associated mass and potential antitumor activity loss with bioactive extracts. Datta S, Zhou YD, and Nagle DG J. Nat. Prod. 76(4), 642-7, (2013)

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FEM1A and FEM1B: novel candidate genes for polycystic ovary syndrome. Goodarzi MO Hum. Reprod. 23(12), 2842-9, (2008)

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Development of a miniature analytical system in a lab-on-valve for determination of trace copper by bead injection spectroscopy Yu, Y.L., et al. Talanta 88, 352-357, (2012)

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A novel flow-through fluorescence optosensor for the sensitive determination of tetracycline. Shen LM, Chen ML, and Chen XW Talanta 85(3), 1285-90, (2011)

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Purification and characterization of glutathione S-transferase from turkey liver and inhibition effects of some metal ions on enzyme activity. Akkemik E, Taser P, Bayindir A, et al. Environ. Toxicol. Pharmacol. 34(3), 888-94, (2012)

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Anti-inflammatory effect of IMMLG5521, a coumarin derivative, on Sephadex-induced lung inflammation in rats. Li Z, Hu J, Sun M, et al. Int. Immunopharmacol. 14(2), 145-9, (2012)

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Qualitative and semi-quantitative analysis of phenolics in Eucalyptus globulus leaves by high-performance liquid chromatography coupled with diode array detection and electrospray ionisation mass spectrometry. Boulekbache-Makhlouf L, Meudec E, Mazauric JP, et al. Phytochem. Anal. 24(2), 162-70, (2013)

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Cryopreservation with α-tocopherol and Sephadex filtration improved the quality of boar sperm. Satorre MM, Breininger E, and Beconi MT Theriogenology 78(7), 1548-56, (2012)

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Skin-healing activity and toxicological evaluation of a proteinase fraction from Carica candamarcensis. Lemos FO, Ferreira LA, Cardoso VN, et al. Eur. J. Dermatol. 21(5), 722-30, (2011)

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Inhibition of human platelet aggregation by eosinophils. Maziero AM, Lorenzetti R, Donato JL, et al. Life Sci. 93(9-11), 416-22, (2013)

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Rapid purification and characterization of γ-glutamyl-transpeptidase from shiitake mushroom (Lentinus edodes). Li J, Huang J, Yin J, et al. J. Food Sci. 77(6), C640-5, (2012)

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Structural and quantitative analysis of antioxidant and low-density lipoprotein-antioxidant flavonoids from the grains of sugary rice. Lee DY, Shrestha S, Seo WD, et al. J. Med. Food 15(4), 399-405, (2012)

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Light effect on the stability of β-lapachone in solution: pathways and kinetics of degradation. Cunha-Filho MS, Estévez-Braun A, Pérez-Sacau E, et al. J. Pharm. Pharmacol. 63(9), 1156-60, (2011)

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Composition and antioxidant activity of water-soluble polysaccharides from Tuber indicum. Luo Q, Zhang J, Yan L, et al. J. Med. Food 14(12), 1609-16, (2011)

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Mass transport at rotating disk electrodes: effects of synthetic particles and nerve endings. Chiu VM, Lukus PA, Doyle JL, et al. Anal. Biochem. 418(1), 58-65, (2011)

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Two-start design within a Sephadex inflammatory model--a means to generate reliable ED50 data whilst significantly reducing the number of animals used. Siller H, Taylor JD, and Middleton B Pulm. Pharmacol. Ther. 25(3), 223-7, (2012)

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Analgesic and antibutyrylcholinestrasic activities of the venom prepared from the Mediterranean jellyfish Pelagia noctiluca (Forsskal, 1775). Ayed Y, Dellai A, Ben Mansour H, et al. Ann. Clin. Microbiol. Antimicrob. 11, 15, (2012)

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Experimental test of absorption flattening correction for circular dichroism of particle suspensions. Gerdova A, Kelly SM, and Halling P Chirality 23(7), 574-9, (2011)

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Molecular weight distribution of organic matter by ozonation and biofiltration. Lin YH Water Sci. Technol. 66(12), 2604-12, (2012)

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Preparation of highly purified C-phycoerythrin from marine cyanobacterium Pseudanabaena sp. Mishra SK, Shrivastav A, and Mishra S Protein Expr. Purif. 80(2), 234-8, (2011)

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Effects of free Ca²⁺ on kinetic characteristics of holotransketolase. Solovjeva ON, Sevostyanova IA, Yurshev VA, et al. Protein J. 31(2), 137-40, (2012)

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