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G2654 Sigma

Monoclonal Anti-Glucagon antibody produced in mouse

clone K79bB10, ascites fluid

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Pancreatic Stem Cells, Antibodies for Stem Cell Biology,
species reactivity   rat, pig, guinea pig, feline, mouse, human, canine, rabbit
application(s)   dot blot: suitable
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:2,000 using human or animal pancreas
  radioimmunoassay: suitable
clone   K79bB10, monoclonal
antibody form   ascites fluid
isotype   IgG1
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... GCG(2641)
mouse ... Gcg(14526)
rat ... Gcg(24952)
conjugate   unconjugated

Description

Immunogen

Polymerized porcine glucagon.

General description

Glucagon is a 29 residue containing peptide hormone secreted by pancreas. The main functions of pancreatic glucagon consist of gluconeogenesis, ketogenesis, lipolysis and glycogenolysis. Monoclonal anti-Glucagon antibody can be used as an analytical tool for quantification of the hormone. It can also be used for immunocytochemical staining of formalin fixed and Bouin-fixed, paraffin-embedded pancreatic tissue sections. Mouse anti-Glucagon antibody reacts specifically with pancreatic glucagon. The product has also shown cross reactivity with glucagon-containing cells in fixed sections of pancreas from dog, mouse, rat, rabbit, porcine, guinea pig, cat and human and weak cross reactivity for gut glucagon (enteroglucagon).

Specificity

Monoclonal Anti-Glucagon reacts with pancreatic glucagon in RIA and immunocytochemistry. The affinity constant of 6.1 x 10(8) L/M in RIA. The antibody weakly cross-reacts with gut glucagon (enteroglucagon) in an immunohistological assay. Cross-reactivity has been observed with glucagon-containing cells in fixed sections of pancreas from human, porcine, dog, rabbit, mouse, rat, guinea pig, and cat.

The antibody reacts specifically against pancreatic glucagon and exhibits only very weak cross-reaction with gut glucagon (enteroglucagon).1 May be used for the immunohistochemical staining of Bouin′s-fixed, and formalin-fixed, paraffin-embedded pancreatic tissue sections. Binds to glucagon with an affinity constant of 6.1 × 108 M-1 in RIA.1

Application

Monoclonal anti-Glucagon antibody can be used in immunocytochemistry1; RIA and dot blot technique (native and denatured). It can also be used in immunohistochemistry (diluted 1:5,000) 2.

Features and Benefits

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Price and Availability


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