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G2781 Sigma

Anti-Glutamine Synthetase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Neurobiology, Antibodies for Neurotransmission, Antibodies for Neurotransmitter Synthesis and Metabolism,
species reactivity   rat
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:10,000 using rat brain sections
  microarray: suitable
  western blot: 1:10,000 using rat brain cytosolic fraction
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 45 kDa
storage temp.   −20°C
Gene Information   human ... GLUL(2752)
mouse ... Glul(14645)
rat ... Glul(24957)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to the C-terminal region of mouse glutamine synthetase (GS) (amino acids 357-373. This sequence is identical in human, bovine, rat, hamster, and pig GS and highly conserved chicken
Gs (single amino acid substitution).

General description

Glutamine synthetase (GS) catalyzes the amidation of glutamate to form glutamine. It is found in mammals as an octamer of identical 45 kDa subunits. In the brain, GS is considered a key enzyme participating in the recycling of the excitatory neurotransmitter glutamate released from neurons and termination of the neurotransmitter signal, as well as in ammonia detoxification.
GS is localized in glial cells of various species. It is present in the brain primarily in astrocytes and in the retina in Muller glial cells that support neurons using glutamate as a neurotransmitter. GS activity is a useful marker for astrocytes, and an important differentiation feature in the retina. GS is found in several brain regions including hippocampus, cerebral cortex, neostriatum and cerebellar granular layer, corresponding to sites with high glutamate receptor density. Upregulation of GS is a hallmark of reactive astrocytosis and GS activity level increases following spinal cord injury. The GS expression in astrocytes is diminished in brains affected by Alzheimer′s disease, particularly in the vicinity of senile plaques. GS is upregulated in a subset of human hepatocellular carcinomas, suggesting that its expression may be related to tumor recurrence, since tumors expressing GS are independent of the extracellular glutamine supply.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Specificity

Anti-Glutamine synthetase recognizes rat glutamine synthetase (GS) (45 kDa). Staining of GS in immunoblotting is specifically inhibited with the GS immunizing peptide (amino acids 357-373 with Nterminally added lysine).

Application

Anti-Glutamine Synthetase antibody is suitable for detection and localization of GS by immunoblotting and immunohistochemistry.
Immunoblotting: a minimum working antibody dilution of 1:10,000 is determined using a rat brain cytosolic fraction extract.
Immunohistochemistry: a minimum working antibody dilution of 1:10,000 is determined using formalin-fixed, paraffin-embedded sections of rat brain.

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Safety Information

RIDADR  NONH for all modes of transport
Protocols & Articles

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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