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G2N350 Sigma

GenElute Plant Genomic DNA Miniprep Kit Green Alternative

sufficient for 350 purifications

Synonym: Gen Elute, Plant Genomic DNA Miniprep

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Properties

Related Categories 12 Principles Aligned Products, Agriculture, Cell Biology, Chemical Synthesis, DNA & RNA Purification,
usage   sufficient for 350 purifications
greener alternative product characteristics   Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.
storage temp.   room temp

Description

Features and Benefits

• Starting material: Up to 100 mg of plant tissue
• Expected yield: Up to 20 μg
• Elution volume: 100 - 200 μl
• Time required: < 40 min
• RNase treatment required: No

General description

Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

The GenElute Plant Genomic DNA Miniprep Kit provides a simple and convenient way to isolate pure DNA from a variety of plant species. The GenElute kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, RNase treatment, and hazardous organic compounds such as phenol and chloroform.

Other Notes

For additional information, please see www.sigma-aldrich.com/genomicdna.

Application

The purified genomic DNA is ready for immediate use in sensitive downstream applications such as:
• PCR
• restriction endonuclease digestion
• cloning
• Southern blots

Principle

Several micrograms of DNA can be obtained from up to 100 mg of fresh tissue or 10 mg of freeze-dried material in less than an hour. Plant tissue is disrupted by grinding in liquid nitrogen and the DNA is released with detergent and chaotrope. Proteins, polysaccharides, and cell debris are eliminated with a 10 minute precipitation procedure followed by centrifugation through a filtration column, included in the kit. The genomic DNA is purified further by a silica bind-wash-elute procedure in microcentrifuge spin columns. The purified DNA is greater than 20 kb in length.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Price and Availability


Microcon Centrifugal Filters

Discover the Milli-Q® IQ 7000 ultrapure water system

Kit component also available separately

Description

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Column Preparation Solution SDS C2112

Kit component only

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Lysis buffer for GenElute plant genomic DNA kit SDS    
Lysis Solution Part B SDS    
Precipitation solution for GenElute plant genomic DNA kit SDS    
Binding solution for GenElute plant genomic DNA kit SDS    
Wash solution for GenElute plant genomic DNA kit SDS    
TE Buffer for GenElute Plant Genomic DNA Kit SDS    
Filtration Column SDS    
Nucleic Acid Binding Column SDS    
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Supplemental Hazard Statements 
Contact with acids liberates very toxic gas.
RIDADR 
UN 3316 9
Protocols & Articles

Protocols

Experienced User Protocol GenElute Plant Genomic DNA Miniprep Kit Protocol

For experienced users, an abbreviated protocol is listed below.  For data, images, troubleshooting and other information, please see the full length protocol page.
Keywords: Cell disruption, MSDS, PAGE

GenElute Plant Genomic DNA Miniprep Kit Protocol (G2N10, G2N70, G2N350)

Several micrograms of DNA can be obtained from up to 100 mg of fresh tissue or 10 mg of freeze-dried material in less than an hour. The purified DNA is greater than 20 kb in length and can be used in...
Keywords: AGE, Amplification, Cell disruption, Centrifugation, Electrophoresis, Evaporation, Filtration, Gel electrophoresis, Molecular biology, Phase transitions, Polymerase chain reaction, Precipitation, Purification

Peer-Reviewed Papers
15

References

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G2N10 GenElute Plant Genomic DNA Miniprep Kit, sufficient for 10 purifications
G2N70 GenElute Plant Genomic DNA Miniprep Kit, sufficient for 70 purifications
C2112 Column Preparation Solution

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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