• USA Home
  • G3011 - Glutathione Reductase from spinach

G3011 Sigma

Glutathione Reductase from spinach

Type VI, ammonium sulfate suspension, 100-200 units/mg protein

Synonym: GR, NAD(P)H: oxidized-glutathione oxidoreductase



Related Categories 1.6.x.x Acting on NAD or NADP, 1.x.x.x Oxidoreductases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
type   Type VI
form   ammonium sulfate suspension
mol wt   mol wt 118 kDa
foreign activity   NADPH oxidase ≤0.01%
storage temp.   2-8°C


Biochem/physiol Actions

A crucial flavoenzyme in the antioxidant defense system. Reduced glutathione (GSH) is used by glutathione peroxidase to detoxify hydrogen peroxide and in the process is converted to oxidized glutathione (GSSG). The GSSG is then recycled back to GSH by glutathione reductase (GR) using NADPH that is then converted to NADP+. The regenerated GSH is then available to detoxify more hydrogen peroxide. The enzyme uses FAD as a cofactor. GR and glutathione peroxidase may inhibit lipid peroxidation by functioning as antioxidant enzymes in sperm. Glutathione reductase shares a structural motif with a number of other proteins including aspartyl proteases, citrate synthase, EF hands, hemoglobins, lipocalins, and α/β hydrolases. GR is stimulated by melatonin and is reportedly irreversibly inhibited by a number of oxygen radical generating systems.

Unit Definition

One unit will reduce 1.0 μmole of oxidized glutathione per min at pH 7.6 at 25°C. (Protein determined by biuret).

Physical form

Suspension in 3.6 M (NH4)2SO4 mM, 10 mM potassium phosphate, pH 7.0

Price and Availability

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

GHS08  GHS08
Signal word 
Hazard statements 
Precautionary statements 
NONH for all modes of transport
WGK Germany 
Protocols & Articles


Ammonium Sulfate Suspensions - Technical Note

Note that for an ammonium sulfate suspension, most of the enzyme will be in solid form. It is likely that only negligible amounts of enzyme will be in the ammonium sulfate solution.
Keywords: Enzyme activity

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers


Related Products

comparable product

Product #


Add to Cart

G3664 Glutathione Reductase from baker's yeast (S. cerevisiae), ammonium sulfate suspension, 100-300 units/mg protein (biuret)


Product #


Add to Cart

C0400 Carmustine, ≥98%

related product

Product #


Add to Cart

GRSA Glutathione Reductase Assay Kit

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?