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G3144 Sigma

L-Glutamine Synthetase from Escherichia coli W, ATCC No. 9637

Protein ≥30 % by biuret, lyophilized powder

Synonym: L-Glutamate: ammonia ligase (ADP-forming)

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Properties

Related Categories 6.3.x.x C-N Bonds, 6.x.x.x Ligases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
form   lyophilized powder
activity   100-400 units/mg protein
composition   Protein, ≥30% biuret
solubility   H2O: soluble0.95 - 1.05  mg/mL, clear to slightly hazy
storage temp.   −20°C
Gene Information   Escherichia coli K12 ... glnA(948370)

Description

Biochem/physiol Actions

Degradative enzyme for glutamic acid

L-glutamine synthetase catalyzes the condensation of L-glutamate and ammonia to L-glutamine. It is a degradative enzyme for glutamic acid.

Preparation Note

Grown in medium containing glucose and NH4Cl

Unit Definition

One unit will convert 1.0 μmole of L-glutamate to L-glutamine in 15 min at pH 7.1 at 37 °C.

Physical form

Lyophilized powder containing buffer salts and stabilizer

Application

L-Glutamine synthetase may be used for the purification of proteases from Escherichia coli.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

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