• USA Home
  • G3272 - Guanidine hydrochloride

EMAIL THIS PAGE TO A FRIEND
G3272 Sigma

Guanidine hydrochloride

for molecular biology, ≥99%

Synonym: Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidinium chloride

Purchase

Description

Application

Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.

Guanidine hydrochloride is used in RNA isolation to dissociate nucleoproteins and inhibit RNase.

Caution

May agglomerate upon storage. The quality of the product does not appear to be affected and solutions prepared from the free-flowing and lumpy guanidine hydrochloride appear identical.

Packaging

1, 2 kg in poly bottle

100, 500 g in poly bottle

Preparation Note

In order to make an 8M solution in water, one must heat the solution to 35 °C for approximately 30 minutes.
The maximum solubility of guanidine hydrochloride in water at room temperature is approximately 6M.

Price and Availability


Detect Your Unseen Protein

All labs need water

Refer a colleague - New Lab Start-Up program
Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
MF4300000
Protocols & Articles

Protocols

TRI Reagent® Protocol

1A. Tissue: Homogenize tissue samples in TRI Reagent (1 ml per 50–100 mg of tissue) in a Polytron® or other appropriate homogenizer.
Keywords: AGE, Buffers, Cell disruption, Centrifugation, Degradations, Digestions, Electrophoresis, Evaporation, Gel electrophoresis, Homogenization, PAGE, Polymerase chain reaction, Precipitation, Sample preparations, Separation, Solvents, Western blot

Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?