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G3512 Sigma

Glycerol Dehydrogenase from Cellulomonas sp.

lyophilized powder, 50-125 units/mg protein

Synonym: Glycerol:NAD+ 2-oxidoreductase

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Properties

Related Categories 1.1.x.x Acting on hydroxyl groups, 1.x.x.x Oxidoreductases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
form   lyophilized powder
mol wt   mol wt ~390 kDa
composition   Protein, ≥60% biuret
storage temp.   −20°C

Description

Packaging

250, 1000 units in poly bottle

Unit Definition

One unit will oxidize 1.0 μmole of glycerol to dihydroxyacetone per min at 25 °C at pH 10.0.

Physical form

Lyophilized powder containing bovine serum albumin

Application

This enzyme is useful for enzymatic determination of glycerol and of triglyceride when coupled with lipoprotein lipase in clinical analysis. Formation of NADH from the reaction of glycerol and NAD+ was catalyzed by the enzyme glycerol dehydrogenase.

Biochem/physiol Actions

Glycerol dehydrogenase catalyzes the conversion of glycerol to glycerone.

Physical properties

Isoelectric point : 4.4 ± 0.1
Michaelis constants : 1.1 x 10¯2M (Glycerol), 8.9 x 10¯5M (NAD+)
Structure : 10 subunits (42,000) per mol of enzyme
Inhibitors : p-Chloromercuribenzoate, o-phenanthroline, monoiodoacetate, heavy metal
ions (Co++, Ni++, Cu++, Zn++, Cd++)
Optimum pH : 10.0 – 10.5
Optimum temperature : 50°C
pH Stability : pH 7.5 – 10.5 (25°C, 20hr)
Thermal stability : below 55°C (pH 7.5, 15min)
Substrate specificity : This enzyme has the highest specificity for glycerol and 1,2-propanediol, and also oxidizes glycerol-α-monochlorohydrin, ethylene
glycol and 2,3-butanediol. The oxidative reaction is stimulated by K+, NH+4 and Rb+.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Protocols

Assay Procedure for Glycerol Dehydrogenase

One unit causes the formation of one micromole of NADH per minute under the conditions described below.
Keywords: Extinction coefficient

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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