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G3795 Sigma

Monoclonal Anti-Gravin antibody produced in mouse

clone JP74, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-A-Kinase Anchor Protein 12, Anti-AKAP12

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Properties

Related Categories A-Kinase Anchor Protein 12 (Gravin), Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology,
species reactivity   mouse, rat, human
application(s)   immunocytochemistry: suitable
  immunoprecipitation: suitable
  western blot: 0.02-0.04 μg/mL using rat brain cytosolic S1 fraction
clone   JP74, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG2a
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... AKAP12(9590)
mouse ... Akap12(83397)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

fractionated membrane cortex proteins from human neuron cell lines NT2-N and SK-N-BE(2).

General description

Gravin (or AKAP12) is a scaffolding kinase that functions as a tumor suppressor. This kinase interacts with PKC, PKA and other cell cycle signaling proteins to modulate cell division, growth, differentiation and inflammatory responses. The expression of gravin isoforms (α, β, and γ) is regulated by tissue-specific promoters. Gravin also acts as an autoantigen in myasthenia gravis patients . Monoclonal Anti-Gravin antibody is specific for human, rat and mouse gravin (approx. 300 kDa).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Monoclonal Anti-Gravin antibody is suitable for use in immunoblotting (using mpkCCD cells ). The product may also be used for immunocytochemistry and immunoprecipitation.

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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