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G3893 Sigma

Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) antibody produced in mouse

clone G-A-5, ascites fluid

Synonym: Anti-GFAP

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Neural Stem Cells, Antibodies for Neurobiology,
species reactivity   human, pig, rat
application(s)   immunohistochemistry: 1:400 using rat brain sections (alcohol-fixed)
  microarray: suitable
clone   G-A-5, monoclonal
antibody form   ascites fluid
isotype   IgG1
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... GFAP(2670)
rat ... Gfap(24387)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

GFAP from pig spinal cord

Specificity

The antibody reacts specifically with GFAP in immunoblotting assays and labels astrocytes, Bergmann glia cells and chondrocytes of elastic cartilage in immunohistochemical staining. The antibody reacts with glial specific antigen in frozen or alcohol-fixed tissue sections.

Application

Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) antibody produced in mouse is suitable for immunohistochemistry at a working dilution of 1:400 using rat brain sections (alcohol-fixed) and microarray analysis. It may be used for immunocytochemical localization of GFAP in human, pig, and rat tissues. It is also suitable for localization of GFAP using immunoblot assays. In indirect immunofluorescent labeling on alcohol-fixed or frozen sections, this antibody stains astrocytes and Bergmann glia cells, gliomas, and other glial cell derived tumors.
The antibody was used as a primary antibody in immunocytochemistry analysis:
• of primary cerebral microvascular EC cultures to study the effect of microglia on the BBB (blood-brain barrier) and its primary constituents
• to study the wound healing effects of matrix metalloproteinase-2 that promote recovery after spinal cord injury
• to study the negative regulation of embryonic neural progenitor cell proliferation by Toll-like receptor 3

Features and Benefits

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Biochem/physiol Actions

The gene GFAP encodes an intermediate filament protein (50kDa) of mature astrocytes, which may be used as a marker for distinguishing astrocytes from other glial cells during development of the central nervous system. Defects in this gene causes Alexander disease. It is a rare disorder of astrocytes in the CNS.

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