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  • G4040 - Anti-Gα i3 antibody produced in rabbit

G4040 Sigma

Anti-Gα i3 antibody produced in rabbit

whole antiserum, buffered aqueous solution



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to G Proteins, Antibodies to G Proteins and Cyclic Nucleotides,
species reactivity   hamster, rat, mouse, human
application(s)   immunoprecipitation: suitable
  western blot: 1:1,000-1:2,000 using solubilized mouse and rat brain membrane lysates, and mouse 3T3 cell lysates
clone   polyclonal
antibody form   whole antiserum
form   buffered aqueous solution
mol wt   antigen mol wt ~40 kDa
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... GNAI3(2773)
mouse ... Gnai3(14679)
rat ... Gnai3(25643)
biological source   rabbit
conjugate   unconjugated



synthetic peptide corresponding to the C-terminal region of Gαi3.

General description

G-proteins are membrane associated heterotrimeric proteins that are comprised of α-, β-, and λ-subunits. These proteins play a key role in signal transduction pathways that regulate cell′s response to several hormones, neuromodulators and ligands.

Physical form

Solution of whole antiserum diluted in PBS, pH 7.4.


Recognizes Gαi3 and Gα0 G-proteins.


Anti-Gα i3 antibody produced in rabbit is suitable for immunoprecipitation at a working antibody amount of 4μL using solubilized mouse brain membrane lysates and immunoblotting at a working dilution of 1:2000 using solubilized mouse and rat brain membrane lysates, and mouse 3T3 cell lysates.

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Biochem/physiol Actions

Gi α 3 is a G-protein subunit that is involved in many signal transduction pathways including the mediation of EGF-induced PLC-γ activation and Ca+2 mobilization in hepatocytes. The α-subunit contains a guanine-binding domain that is in its inactive state when it is occupied by GDP. Upon activation, GDP is replaced with GTP, causing the dissociation of the α-subunit from the βλ-subunit complex. This enables the Gα-GTP complex to bind to and regulate specific signaling pathways. GTP is then hydrolyzed, allowing for re-association of the α-subunit with the βλ-subunit complex.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
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