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G4142 Sigma

β-Galactosidase from bovine testes

ammonium sulfate suspension, 1.0-3.0 units/mg protein (modified Warburg-Christian)

Synonym: β-D-Galactoside galactohydrolase

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Properties

Related Categories 3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Carbohydrate Hydrolysis,
form   ammonium sulfate suspension
foreign activity   α- and β-glucosidase ≤0.2%
  α-fucosidase ≤0.2%
  α-galactosidase ≤0.2%
  α-mannosidase ≤0.2%
  β-N-acetylglucosaminidase ≤1.0%
storage temp.   2-8°C

Description

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrophenyl β-D-galactopyranoside to p-nitrophenol and D-galactopyranose per min at pH 4.4 at 25 °C.

Physical form

Suspension in 3.2 M (NH4)2SO4, pH approx. 5.0

Application

β-Galactosidase from bovine testes was used for coupling to Sepharose.

Biochem/physiol Actions

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

Price and Availability


Amplified Detection

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
LW5840000
Protocols & Articles

Articles

Ammonium Sulfate Suspensions - Technical Note

Note that for an ammonium sulfate suspension, most of the enzyme will be in solid form. It is likely that only negligible amounts of enzyme will be in the ammonium sulfate solution.
Keywords: Enzyme activity

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

Related Products

inhibitor

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Description

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D6518 Diethylenetriaminepentaacetic acid, ≥99% (titration)
P9375 1,10-Phenanthroline monohydrate, reagent grade
E4378 Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid, ≥97.0%
ED2SS Ethylenediaminetetraacetic acid disodium salt dihydrate, reagent grade, 98.5-101.5% (titration)

related product

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08168 Timestrip Plus 8 °C

substrate

Product #

Description

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M1633 4-Methylumbelliferyl β-D-galactopyranoside, ≥99% (TLC)
B4252 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, ≥98%, powder
B6024 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, tablet
F2756 Fluorescein di(β-D-galactopyranoside)
B4387 5-Bromo-3-indolyl β-D-galactopyranoside, ≥98%
N9643 Naphthofluorescein di-(β-D-galactopyranoside), ≥95%

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