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G4155 Sigma

β-Galactosidase from Escherichia coli

aqueous glycerol suspension, 600-1,200 units/mg protein (biuret)

Synonym: β-D-Galactoside galactohydrolase, Lactase

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Description

Other Notes

Loss of enzymatic activity can occur if this enzyme is frozen. This enzyme is provided in a 50% glycerol solution and will not freeze if stored at -20° Celsius. It is not recommended that this enzyme preparation be stored in an ultracold freezer (-60 to -100° Celsius).

Packaging

5000, 15000 units in glass bottle

Unit Definition

One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.

Physical form

Suspension in 50% glycerol, 5 mM Tris buffer salts, 5 mM magnesium chloride, 0.5 mM DTT, 0.5 mM mercaptoethanol. pH 7.4

Application

β-Galactosidase is used in the enzymatic assays in the synthesis of imidazolo-pyrrolidinoses.

Biochem/physiol Actions

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

Physical properties

Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)

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Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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substrate

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M1633 4-Methylumbelliferyl β-D-galactopyranoside, ≥99% (TLC)
B4252 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, ≥98%, powder
B6024 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, tablet
F2756 Fluorescein di(β-D-galactopyranoside)
B4387 5-Bromo-3-indolyl β-D-galactopyranoside, ≥98%
N9643 Naphthofluorescein di-(β-D-galactopyranoside), ≥95%

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