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G4252 Sigma

Glyoxalase I from Saccharomyces cerevisiae

Grade IV, buffered aqueous glycerol solution, ≥400 units/mg protein

Synonym: S-Lactoyl-glutathione methylglyoxal-lyase (isomerizing)

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Properties

Related Categories 4.4.x.x C-S bonds, 4.x.x.x Lyases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
type   Grade IV
form   buffered aqueous glycerol solution
foreign activity   glyoxalase II ≤1%
storage temp.   2-8°C

Description

Analysis Note

Protein determined by biuret.

Biochem/physiol Actions

Glyoxalase I is universally expressed and involved in the protection against cellular damage due to cytotoxic metabolites such as advanced glycation end products (AGEs). It is an integral component of the detoxification system, catalyzing the conversion of reactive, acyclic a-oxoaldehydes into the corresponding a-hydroxyacids in a glutathione-dependent manner.

Packaging

500, 1000, 2500 units in glass bottle

Unit Definition

One unit will form 1.0 μmole of S-lactoylglutathione from methylglyoxal and reduced glutathione per min at pH 6.6 at 25 °C.

Physical form

Solution in 50% glycerol, 0.4 M (NH4)2SO4 and 0.002 M KH2PO4 pH 6.5

General description

Glyoxalase detoxification system consists of glyoxalase (GLO)-I and GLO-II. GLO-I is a cytosolic, 42 kDa, dimeric Zn2+ metalloenzyme.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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