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G4256 Sigma

Galactose-1-phosphate Uridyltransferase from galactose-adapted yeast

Type IV, lyophilized powder, 20-60 units/mg protein (modified Warburg-Christian)

Synonym: GALT, UDP glucose:α-D-galactose-1-phosphate uridyltransferase

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Properties

Related Categories 2.7.x.x Phosphorus containing groups, 2.x.x.x Transferases, Biochemicals and Reagents, Carbohydrate Metabolism, Cell Biology,
Quality Level   PREMIUM
type   Type IV
form   lyophilized powder
composition   Protein, 15-35%
foreign activity   6-phosphogluconate dehydrogenase ≤0.5%
  UDP glucose pyrophosphorylase and galactokinase ≤0.2%
storage temp.   −20°C

Description

Biochem/physiol Actions

Galactose-1-phosphate uridyltransferase (GALT) facilitates the simultaneous conversion of uridine diphosphoglucose (UDP-glucose) and galactose-1-phosphate (gal-1P) to uridine diphosphogalactose (UDP-galactose) and glucose-1-phosphate. Classic Galactosemia (CG) is an inherited metabolic condition caused by deficiency of GALT activity.

Packaging

20, 50 units in glass bottle

Unit Definition

One unit will form 1.0 μmole of glucose 1-phosphate from UDP-glucose, galactose 1-phosphate and NADP+ per min at pH 8.7 at 25 °C as detected by a coupled system using phosphoglucomutase.

Physical form

Contains buffer salts as citrate and reduced glutathione

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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