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G4259 Sigma

β-Glucuronidase from Helix aspersa (garden snail)

Type HA-4

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Properties

Related Categories β-Glucuronidase, 3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Analytical and Industrial Enzymes, Application Index,
type   Type HA-4
form   partially purified powder
activity   ≥300,000 units/g solid solid
secondary activity   ≤7,500 units/g solid sulfatase
solubility   H2O: soluble1.90 - 2.10  mg/mL, clear to slightly hazy
Featured Industry   Clinical Testing
storage temp.   −20°C

Description

Other Notes

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Packaging

1000000 units in poly bottle

Unit Definition

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37°C at the pH 5.0 (30 min assay).

One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.

Application

β-glucuronidase was used in enzymic hydrolysis of tissue homogenates for liquid chromatography-electrospray ion trap mass spectrometry (LC/MSn) analysis, to study the structures of degradation products of baicalin.

Biochem/physiol Actions

β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.

Price and Availability

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
LZ8990000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

UHPLC/MS of Drugs and Metabolites in Urine following Enzymatic Hydrolysis

β-D-Glucuronide glucuronosohydrolase enzymes, commonly shortened to β-glucuronidase, play an important role in the analysis of biological fluids for the presence of metabolites for drug screening and...
Craig Aurand, Supelco; Kristen Brown, Pennsylvania State University
Reporter US Volume 33.2
Keywords: Carbohydrate metabolism, Derivatizations, Digestions, Enzyme activity, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolism, Metabolites, Sample preparations, Separation, Solid phase extractions, Solvents

Protocols

Enzymatic Assay of ß-Glucuronidase (EC 3.2.1.31) from Helix Pomatia and Bovine Liver

To standardize a procedure for Enzymatic Assay of b-Glucuronidase1 at Sigma-Aldrich St. Louis.
Keywords: Enzymology

Related Content

Analytical Enzyme Beta Glucuronidase

Sigma® β-Glucuronidases are routinely used for the enzymatic hydrolysis of glucuronides from urine1,2, plasma3,4, and other fluids5 prior to analysis by enzyme immunoassay, mass spectrometry, gas chr...
Keywords: Bacterial conjugations, Cancer, Chromatography, Gas chromatography, High performance liquid chromatography, Immunoassay, Mass spectrometry, PAGE, Purification

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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