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G4280 Sigma

Anti-Guanylyl Cyclase α1 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Stress, Antibodies for Nitric Oxide Research,
species reactivity   rat, mouse, bovine, human
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using trypsin-treated human, bovine and mouse heart tissue
  immunoprecipitation: 5-10 μg using 60-120 μg of a cytosolic fraction of rat brain
  western blot: 1:10,000 using cytosolic fraction of rat brain
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   mol wt ~80 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... GUCY1A3(2982)
mouse ... Gucy1a3(60596)
rat ... Gucy1a3(497757)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acid residues 673-690 of rat soluble Guanylyl cyclase α1, conjugated to KLH with glutaraldehyde. The corresponding human sequence differs by 2 amino acids.

General description

Guanylyl cyclase (GC) catalyzes the conversion of guanosine-5’–triphosphate (GTP) to cyclic guanosine-3’,5-monophosphate (cGMP) and pyrophosphate. This reaction requires Mg2+ or Mn2+. The enzyme consists of α and β subunits of ~80kDa and ~70kDa respectively. Both the units are required for catalytic activity. The N-terminal domains of the subunits are essential for the stimulation of the enzyme by NO. Dimerization is mediated by the central portion of GC. The C-terminus domain of both subunits forms the catalytic domain.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Application

Anti-Guanylyl Cyclase α1 antibody produced in rabbit is suitable for immunohistochemistry at a working dilution of 1:100 using trypsin-treated human, bovine and mouse heart tissue and immunoprecipitation at 5-10μg concentration using 60-120μg of a cytosolic fraction of rat brain. It is also suitable for western blot analysis using cytosolic fraction of rat brain at a working dilution of 1:10,000.
It was used as a primary antibody for immunohistochemical:
• localization of α1 subunits of sGC (soluble guanylate cyclase) in the guinea pig gastrointestinal tract
• detection of expression of sGC in the vasculature of rat skeletal muscle
• localization of the functional subunit of NO receptors, sGCα1 in guinea pig caecum

Features and Benefits

Antibody Bioguarantee
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Biochem/physiol Actions

The enzyme (GC) is a major physiological receptor for nitric oxide (NO), an important intra- and intercellular membrane-permeant signaling molecule. Gaseous NO binds to Fe2+ in the prosthetic heme group of the enzyme. NO binding is followed by disruption of the β1 subunit histidine105 bond to iron and activation of the enzyme. GC forms a complex with NO and cGMP and regulates smooth muscle relaxation, inflammation, platelet adhesion and aggregation, pulmonary physiology and neuronal function. It is an important target for NO-releasing and non-NO-releasing activator drugs in human cardiovascular therapy.

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