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  • G4290 - Anti-Gαq11, C-Terminal antibody produced in rabbit

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G4290 Sigma

Anti-Gαq11, C-Terminal antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to G Proteins, Antibodies to G Proteins and Cyclic Nucleotides,
species reactivity   human, mouse
application(s)   immunoprecipitation: suitable
  western blot: 1:500-1:2,000 using rat brain microsomal preparation (20 μg)
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... GNAQ(2776)
mouse ... Gnaq(14682)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 350-359 of Gαq11 or amino acids 344-353 of Gαq.

General description

G-proteins are membrane associated heterotrimeric proteins comprising of α-, β-, and γ-subunits. The α-subunit contains a guanine-binding domain that is in an inactive state when it is occupied by GDP. Upon activation, GDP is replaced with GTP, causing the dissociation of the α-subunit from the βγ-subunit complex. This enables the Gα-GTP complex to bind to and regulate specific signaling pathways. GTP is then hydrolyzed, allowing for re-association of the α-subunit with the βγ-subunit complex.

Physical form

Solution in phosphate buffered saline, pH 7.4.

Specificity

The sequence is highly conserved among species. The antibody is specific for Gαq11 and Gαq.

Application

Anti-Gαq11, C-terminal antibody produced in rabbit is suitable for immunoprecipitation and western blot analysis at a working dilution of 1:500-1:2000 using 20μg of rat brain microsomal preparation.

Features and Benefits

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Biochem/physiol Actions

Gq α (G11α) regulates the action of phospholipase C (PLC), and results in the release of other intracellular messengers through the inositol phosphate pathway, leading to the release of Ca2+ and the activation of phosphorylation.

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Safety & Documentation

Documents

Certificate of Analysis

Certificate of Origin

Safety Information

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Protocols & Articles

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Antibody Explorer | Buy Primary & Secondary Antibodies

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
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References

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