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G4296 Sigma

GSK3β, active, His tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

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Properties

Related Categories Active Kinases, Alzheimer′s Disease, Application Index, Biochemicals and Reagents, Cell Biology,
recombinant   expressed in baculovirus infected Sf9 cells
grade   PRECISIO® Kinase
assay   ≥70% (SDS-PAGE)
form   buffered aqueous glycerol solution
activity   143-193 nmol/min·mg
mol wt   mol wt ~48 kDa
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... GSK3B(2932)

Description

Physical form

Supplied in 50mM NaPhosphate pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1mM PMSF, 0.2 mM DTT, 25% glycerol.

Biochem/physiol Actions

GSK3β is a serine threonine protein kinase that was originally identified as the kinase that phosphorylates and inhibits glycogen synthase.GSK3β is ubiquitously present in human tissues and implicated in the regulation of several physiological processes, including the control of glycogen and protein synthesis by insulin and modulation of the transcription factors AP-1 and CREB. Transient transfection of human GSK3β into Chinese hamster ovary cells stably transfected with individual human tau isoforms leads to hyperphosphorylation of tau at all the sites investigated with phosphorylation-dependent anti-tau antibodies.

Glycogen Synthase Kinase 3β (GSK3β) is responsible for rapid cyclin D1 turnover by phosphorylating it on Thr-286. It functions as a negative regulator of Wnt signaling during embryonic development and cell proliferation in adult tissues. This protein phosphorylates and regulates multiple proteins, such as glycogen synthase, insulin receptor substrate-1, axin and APC (adenomatous polyposis coli). This protein is also shown to interact with multiple proteins which are linked with Alzheimer’s disease (AD).

General description

GSK3B (glycogen synthase kinase 3β) is one of the two isoforms of GSK3 enzyme, both of which are encoded by different genes. This protein is predominantly cytoplasmic, but is also found to be localized to nucleus and mitochondria. It is a protein-serine kinase. This protein contains a protein kinase domain in its core, and is composed of 482 amino acids with a molecular weight of 46,712Da. It shows a wide range of tissue expression with the highest expression in brain.

Legal Information

PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability


All labs need water

Biomedical Applications
Protocols & Articles

Articles

Alzheimer's Disease

Alzheimer's disease (AD) is the most common cause of dementia in the elderly and is characterized by gradual loss of cognitive functions. Hallmark pathohistological findings of AD include widespread ...
Carolyn L. Crankshaw
BioFiles v7 n2, 2011, 4–8
Keywords: Alzheimer Disease, Gene expression, Genetic, Genetics, Inflammation, Metabolism, Phosphorylations

Mitochondrial Dysfunction and Metabolic Defects

Defects in mitochondrial function are associated with insulin resistance in both human and animal studies. Insulin resistance is associated with a decrease in the number, oxidative capacity, size, an...
Keywords: Acetylations, Apoptosis, Biofiles, Cancer, Carboxylations, Catalog, Catalysis, Citric Acid Cycle, Decarboxylations, Degradations, Diabetes, Enzyme-linked immunosorbent assay, Glycolysis, Immunofluorescence, Immunohistochemistry, Lipid Metabolism, Metabolism, Metabolites, Neurotransmitters, Obesity, Oxidations, Phosphorylations, Physiological Processes, Protocols, Transcription, Transduction, Transfection, Western blot

Neuropsychiatric Disorders and Cell Signaling

Schizophrenia, the most common psychotic disorder, affects ~ 25 million people worldwide and is one of the leading causes of years lived with disability.1 Historically, antipsychotic drug discovery h...
Carolyn Crankshaw, Product Specialist, Sigma® Life Science
Biofiles, Vol. 8, No. 10
Keywords: Degradations, Diseases, Drug discovery, Fractionation, Gene expression, Genetic, Neurodegenerative Diseases, Neurotransmission, Schizophrenia

Peer-Reviewed Papers
15

References

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