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G4410 Sigma

Griess reagent (modified)

Synonym: Greiss

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Reagent for the one step analysis of nitrite.
Griess reagent has been used for measuring nitric oxide (NO) production by evaluating the nitrite content in culture media.

Packaging

10 g in poly bottle

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
When using Product G4410, Griess' reagent (modified), what product should I use to make a nitrite standard curve?
Sodium nitrite (Product No. S2252 or 237213) can be used to make a standard curve. A typical standard curve would cover the range of 0-100 microMolar nitrite.
Will phenol red interfere with the Griess reaction Product G4410, Griess' reagent (modified)?
While phenol red does not interfere with the Griess reaction, it will interfere with the absorption reading taken at 540nm.  It is recommended to use media without phenol red to avoid any interference in the absorption readings.  
Is there an enzymatic method to convert nitrate to nitrite?
A coupled assay to convert nitrate to nitrite has been published by Grisham, M.B., et al., Quantitation of nitrate and nitrite in extracellular fluids. Methods Enzymol., 268, 237-246 (1996).  For this protocol, we recommend using Product No. N7265 (Nitrate Reductase), Product No.L1378 (L-Lactic Dehydrogenase), Product No.N1630 (NADPH), Product No. P8574 (Sodium pyruvate) and Product No. F6625 (Flavin adenine dinucleotide disodium salt).
What is the difference between Product G4410 Griess reagent (modified), and Product 03553, Griess reagent?
There are many formulations of the Griess Reagent.  The formulations for these products are similar; both are proprietary.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles
Peer-Reviewed Papers
15

References

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