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  • G4416 - Anti-GABAA Receptor (α1 subunit) antibody produced in rabbit

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G4416 Sigma

Anti-GABAA Receptor (α1 subunit) antibody produced in rabbit

affinity isolated antibody

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Neurotransmission, Antibodies to GABA and Glycine Receptors,
species reactivity   rat, mouse, human (predicted), bovine (predicted), chicken (predicted)
application(s)   immunohistochemistry: suitable using rat brain sections
  western blot: 1:400-1:1,000 using rat brain membranes
clone   polyclonal
antibody form   affinity isolated antibody
form   lyophilized powder
mol wt   antigen mol wt ~50 kDa
storage temp.   −20°C
Gene Information   human ... GABRA1(2554)
mouse ... Gabra1(14394)
rat ... Gabra1(29705)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

peptide corresponding to amino acids 28-43 of rat GABA(A) receptor α1 subunit.

General description

GABAA and GABAB receptors differ with regard to their ionic characteristics and pharmacological properties. The GABAA receptor is an ionotropic receptor that forms the GABA gated chloride channel and consists of several heterogeneous subunits with membrane recognition sites for benzodiazapenes.

Physical form

Lyophilized from phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 0.05% sodium azide.

Preparation Note

Reconstitute the 0.05 mL vial with 50 μL deionized water. Reconstitute the 0.2 mL vial with 0.2 mL deionized water. After reconstitution, the antibody concentration is ~0.8 mg/mL.

Application

Anti-GABAA Receptor (α1 subunit) antibody produced in rabbit is suitable for detection of GABA-A α1 subunit by immunohistochemistry using rat brain sections and by immunoblotting at a working dilution of 1:400-1:1000 using rat brain membranes.

Features and Benefits

Antibody Bioguarantee
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Biochem/physiol Actions

The inhibitory neurotransmitter GABA signals through two distinct types of pre- and postsynaptic receptors, GABAA and GABAB. Both GABA receptors can regulate depression of synaptic transmission and be involved in the inhibition controlling neuronal excitability. α1 is a subtype of GABAA receptor expressed selectively by serotonergic and GABAergic neurons. Serotonergic neurons express strong α3 immunoreactivity but, do not show any α1 immunoreactivity. On the contrary, GABAergic neurons express both α1 and α3 subunits.

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