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G4505 Sigma

Guanidine hydrochloride

≥99% (titration), organic base and chaeotropic agent

Synonym: Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidinium chloride

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Properties

Related Categories Biochemicals and Reagents, Chaotropic Agents, Core Bioreagents, Denaturation, Nucleosides and Nucleotides,
Quality Level   ELITE
InChI Key   PJJJBBJSCAKJQF-UHFFFAOYSA-N
assay   ≥99% (titration)
pH   (25 °C, 4.6—6 at 573 g/L)
mp   180-185 °C(lit.)
solubility   H2O: soluble6 M
absorption   A6M/260 nm, H2O ≤0.10

Description

Application

Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.

Guanidine hydrochloride is used in RNA isolation to dissociate nucleoproteins and inhibit RNase.

Caution

May agglomerate upon storage. The quality of the product does not appear to be affected and solutions prepared from the free-flowing and lumpy guanidine hydrochloride appear identical. For free-flowing material, please see our Redi-Dri free-flowing product number RDD001.

Packaging

1 kg in poly bottle

2, 10, 25 kg in poly drum

25, 100, 500 g in poly bottle

Preparation Note

In order to make an 8M solution in water, one must heat the solution to 35 °C for approximately 30 minutes.
The maximum solubility of guanidine hydrochloride in water at room temperature is approximately 6M.

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Price and Availability


REDI-DRI
Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
MF4300000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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G4505 Guanidine hydrochloride, ≥99% (titration), organic base and chaeotropic agent

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