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G4510 Sigma

Glutathione−Agarose

lyophilized powder

Synonym: GSH-agarose, S-linked glutathione agarose

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Properties

Related Categories Affinity Chromatography, GST, Glutathione, Glutathione S-transferase (GST), Molecular Biology,
form   lyophilized powder
extent of labeling   ≥10 μmol per mL gel
matrix   4% cross-linked beaded agarose
matrix activation   epoxy
matrix attachment   sulfur
matrix spacer   12 atoms (10 carbon)
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Affinity chromatography using glutathione-agarose permits rapid, mild, non-denaturing and highly selective purification of proteins containing glutathione binding sequences, such as Glutathione S-Transferase (GST), glutathione peroxidase and glyoxalase I.

For use in affinity purification of glutathione-S-transferase (GST) and GST fusion proteins from cell lysates of various kinds.

Packaging

1, 5, 10, 50 mL in poly bottle

Quantity

One g powder swells to 10.5 to 16.0 mls of gel.

Physical form

Lyophilized powder stabilized with lactose

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How much Product G4510, Glutathione-Agarose, do I receive and how do I swell my powder?
This material is sold by the final volume of gel produced after swelling of the powder.  One gram of powder swells to approximately 14 mL of gel. (Approximately 70 mg of powder swells to 1 mL of gel.)  Swell the lyophilized powder in deionized water (use of some buffers can result in non-recoverable loss of volume) at 200 mL/g. Typically 90 to 95% of swelling occurs within 30 minutes at room temperature, but it may require overnight at 2 to 8 °C for 100% swelling to occur. After swelling, the agarose beads must be washed thoroughly with 10 volumes of water or equilibration buffer (such as PBS) to remove the lactose present in the lyophilized product.  To view this information in the G4510 data sheet, click on the following link: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/7/g4510dat.Par.0001.File.tmp/g4510dat.pdf
How is Product G4510, Glutathione-Agarose, resin conjugated to the glutathione, and what is the binding capacity of the resin?
This product consists of glutathione attached through the sulfur to epoxy activated 4% cross-linked beaded agarose, resulting in a 12 atom spacer. Binding capacity: approx. 5 to 10 mg glutathione S-transferase per mL resin.  To view this information in the G4510 data sheet, click on the following link: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/7/g4510dat.Par.0001.File.tmp/g4510dat.pdf
When using Product G4510, Glutathione-Agarose, how do I store the swollen resin?
After the resin has been swollen, it can be stored in 2 M NaCl at 2 to 8 °C. For long-term storage, a bacteriostatic agent such as 1 mM sodium azide or 0.02% thimerosal should be included. Alternatively, the resin can be relyophilized after addition of lactose. To view this information in the G4510 data sheet, click on the following link:  http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/7/g4510dat.Par.0001.File.tmp/g4510dat.pdf
How do I regenerate Product G4510, Glutathione-Agarose, resin?
Regeneration method: 1. Wash with approximately 5 resin volumes of 0.1 M borate buffer, pH 8.5 containing 0.5 M NaCl (Cleansing Buffer 1). 2. Wash with at least 5 column volumes of deionized water. 3. Wash with at least 5 column volumes of 0.1 M acetate buffer, pH 4.5, containing 0.5 M NaCl (Cleansing Buffer 2). 4. Wash with approximately 5 resin volumes of deionized water. For long-term storage, store in 2 M NaCl containing a bacteriostatic agent. 5. Equilibrate with equilibration buffer before use.  To view this information in the G4510 data sheet, click on the following link: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/7/g4510dat.Par.0001.File.tmp/g4510dat.pdf
What reagents are compatible with Product G4510, Glutathione-Agarose, resin?
• Tris or phosphate buffers (pH 6.5 to 9.5) are typical lysis buffers compatible with glutathione affinity chromatography. • Salt concentrations of up to 1 Molar do not interfere with binding. • Protease inhibitors such as EDTA (Product No. E7889) or PMSF (Product No. P7626) are often included in the lysis buffer. Serine protease inhibitors included in the lysis buffer will not interfere with subsequent thrombin or factor Xa treatment as these inhibitors are removed before the proteolysis step. • The binding of GST to glutathione-agarose is unaffected by 1% Triton X-100, 1% Tween-20, 1% CTAB, 10 mM DTT or 0.03% SDS.  To view this information in the G4510 data sheet, click on the following link:  http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/7/g4510dat.Par.0001.File.tmp/g4510dat.pdf
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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