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G4527 Sigma

Gonadotropin from pregnant mare serum

lyophilized powder, BioXtra, suitable for mouse embryo cell culture

Synonym: Equine gonadotropin, PMSG, Pregnant mare serum gonadotropin, eCG

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Properties

Related Categories Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Chorionic Gonadotropin, Core Bioreagents,
sterility   aseptically processed
product line   BioXtra
form   lyophilized powder
activity   1,500-6,000 IU/mg
mol wt   mol wt ~64 kDa
solubility   saline: soluble1 mL/vial
  H2O: soluble
suitability   suitable for mouse embryo cell culture
shipped in   dry ice
storage temp.   −20°C

Description

Application

Equine Gonadotropin, Pregnant Mares′ Serum (PMS-G) is used in conjunction with Human chorionic gonadotropin (hCG, Product No. C 8554) to superovulate female mice. PMS-G is used to promote egg follicle maturation by mimicking the effects of follicle stimulating hormone(FSH). For optimal egg production, hCG is injected 42 to 48 hours post PMS-G injection and is required for follicle rupture. Reconstitute 1 vial of PMS-G with 1 ml of 0.9% Sodium Chloride Solution (Product No. S 8776) to give a final concentration of 50 IU/ml. Intraperitoneally inject 0.1 ml into each animal. Product is qualified for use in mouse embryo preparation.

Biochem/physiol Actions

Human chorionic gondadotropin (hCG) may be used as an alternative to PMSG to induce ovulation in laboratory animals. When hCG was injected into laboratory animals via intravenous (i.v.), intraperitoneal (i.p.), or subcutaneous (s.c.) methodstet, the effective dose values for 50% of the animals (ED50) per animal and per body weight (kg) in parenthesis were as follows:
Mice: i.v. 0.2 (7.7) I.U.; i.p. 0.3 (11.5) I.U.; s.c. 0.7 (26.9) I.U.
Syrian hamsters: i.v. 1.0 (9.5) I.U.; i.p. 1.8 (17.1) I.U.; s.c. 2.6 (24.8) I.U.
Rats: i.v. 1.3 (4.6) I.U.; i.p. 3.5 (12.3) I.U.; s.c.7.5 (26.3) I.U.

Using PMSG, the ED50 values per animal and per body weight (kg) in parenthesis were as follows:
Mice: i.v. 0.8 (30.8) I.U.; i.p. 2.0 (76.9) I.U.; s.c. 2.8 (107.7) I.U
Syrian hamsters: i.v., 3.6 (34.3) I.U.; i. p., 8.0 (76.2) I.U.; s.c., 13.2 (125.7) I.U.
Rats: i.v., 6.0 (76.8) I.U.; i p., 20.8 (73.0) I.U.; s.c., 76.8 (269.5) I.U.

Two chain glycoprotein hormone; stimulates the growth of ovarian follicles and the formation of the corpus luteum.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
TU4517000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Creating Transgenic Mice using CRISPR-Cas9 Genome Editing

While several genome editing tools have been developed in recent years, including zinc finger-based strategies and TALENs (transcription activator-like effector nucleases), none have been as efficien...
Keywords: Amplification, Cell culture, Enzyme-linked immunosorbent assay, Gene expression, Genetic, Genetics, Immunohistochemistry, Immunology, Nutrition, Polymerase chain reaction, Recombination, Southern blot, Transcription, Transfection, Western blot

Peer-Reviewed Papers
15

References

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