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  • G4530 - Anti-Guanylyl Cyclase β1 (SN-15) antibody produced in rabbit

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G4530 Sigma

Anti-Guanylyl Cyclase β1 (SN-15) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Stress, Antibodies for Nitric Oxide Research,
species reactivity   human, rat
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using trypsin-digested human heart tissue
  immunoprecipitation: 2-3 μg using 60-120 μg of a cytosolic fraction of rat brain
  western blot: 1:4,000 using a cytosolic fraction of rat brain
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt 70 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... GUCY1B3(2983)
mouse ... Gucy1b3(54195)
rat ... Gucy1b3(25202)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acid residues 605-619, with N-terminal added lysine, of rat soluble guanylate cyclase β1, conjugated to KLH with glutaraldehyde The sequence differs in human, bovine, and mouse by 3, 1, and 2 amino acids.

General description

Guanylyl cyclase (GC) catalyzes the conversion of guanosine-5’–triphosphate (GTP) to cyclic guanosine-3’,5-monophosphate (cGMP) and pyrophosphate. This reaction requires Mg2+ or Mn2+ . The enzyme consists of α and β subunits of ~80kDa and ~70kDa respectively. Both the units are required for catalytic activity. The N-terminal domains of the subunits are essential for the stimulation of the enzyme by NO. Dimerization is mediated by the central portion of GC. The C-terminus domain of both subunits forms the catalytic domain.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Application

Anti-Guanylyl Cyclase β1 (SN-15) antibody produced in rabbit is suitable for immunoblotting at a working dilution of 1:4000 using a cytosolic fraction of rat brain, immunoprecipitation at a working antibody amount of 2-3μg using 60-120μg of a cytosolic fraction of rat brain and for immunohistochemistry at 1:200 working dilution using trypsin-digested human heart tissue.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

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Biochem/physiol Actions

The enzyme (GC) is a major physiological receptor for nitric oxide (NO), an important intra- and intercellular membrane-permeant signaling molecule. Gaseous NO binds to Fe2+ in the prosthetic heme group of the enzyme. NO binding is followed by disruption of the β1 subunit histidine105 bond to iron and activation of the enzyme. GC forms a complex with NO and cGMP and regulates smooth muscle relaxation, inflammation, platelet adhesion and aggregation, pulmonary physiology and neuronal function. It is an important target for NO-releasing and non-NO-releasing activator drugs in human cardiovascular therapy.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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