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G4896 Sigma

Monoclonal Anti-Gelsolin antibody produced in mouse

clone GS-2C4, ascites fluid

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Actin-associated Proteins/Myosin, Antibodies to Cell and Organelle Proteins,
species reactivity   pig, bovine, human, rabbit
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using heat-induced antigen retrieval sections
  immunohistochemistry (frozen sections): suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: 1:1,000 using human plasma
clone   GS-2C4, monoclonal
antibody form   ascites fluid
isotype   IgG1
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... GSN(2934)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

human plasma gelsolin.

Antigen Background

Gelsolin is an actin-severing protein found in mammalian cells and blood plasma. Has tumor suppressor activity in several cancer cell lines and in nude mice.

Specificity

The antibody is specific for an epitope located on the 47 kDa peptide derived from a chymotryptic cleavage of gelsolin. The antibody reacts with plasma and cytoplasmic gelsolin. An additional application for this antibody is immunoaffinity chromatography, for purification of gelsolin, and for detection of gelsolin-actin complexes in cell extracts.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Monoclonal Anti-Gelsolin antibody produced in mouse is suitable as a primary antibody in western blotting
• for the detection of gelsolin lysates and supernatants of UVB-irradiated keratinocytes to examine whether caspase-4 expression is required for UVB-induced unconventional protein secretion
• to measure plasma gelsolin levels
It is suitable for immunoblotting at a working dilution of 1:1000 using human plasma extract. It may be used for ELISA and immunofluorescent staining of frozen sections for detection of gelsolin-actin complexes. This antibody has also been used in immunoaffinity chromatography for purification of gelsolin and for detection of gelsolin-actin complexes in cell extracts.

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Biochem/physiol Actions

Gelsolin is a calcium dependent actin binding protein. It is a potent modulator of actin filament length and gelation. It binds to the ′plus′ ends of actin monomers and filaments, preventing monomer exchange. It exists in two variant forms, cytoplasmic gelsolin and plasma gelsolin. Plasma gelsolin is also called actin depolymerizing factor (ADF) or brevin. Mutations in this gene cause familial amyloidosis Finnish type (FAF) characterized by cranial neuropathy and lattice corneal dystrophy. Gelsolin is used as a biochemical marker for acute rheumatic carditis.

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  nwg
Protocols & Articles

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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